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High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR amplified DNA

High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR... We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Cellular Pathology IOS Press

High resolution comparative genomic hybridization detects 7-8 megabasepair deletion in PCR amplified DNA

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Publisher
IOS Press
Copyright
Copyright © 2001 by IOS Press, Inc
ISSN
0921-8912
eISSN
1878-3651
Publisher site
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Abstract

We investigated if any change in spatial resolution of comparative genomic hybridization analysis could be detected when using DNA amplified by degenerate oligonucleotide primed PCR (DOP-PCR) as opposed to the use of unamplified DNA. Five DNA samples from B-cell leukemias with small 11q deletions were amplified by DOP-PCR and analysed by means of high resolution comparative genomic hybridization (HR-CGH) for the evaluation of aberration size detection limit. By means of HR-CGH, we found the detection limit of DOP-PCR CGH for deletions to be between 3 Mbp and 7-8 Mbp.

Journal

Analytical Cellular Pathology – IOS Press

Published: Jan 1, 2001

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