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Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally twinned crystals of rubredoxin oxygen oxidoreductase from Desulfovibrio gigas

Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally... Crystals of rubredoxin oxygen oxidoreductase have been obtained and characterized. They belong to space group P21212, with unit-cell dimensions a = 88.24 (15), b = 101.25 (7), c = 90.80 (3) A. The homodimer (86 kDa) in the asymmetric unit is related by a non-crystallographic twofold rotation axis parallel to the ab `diagonal' direction, as shown by the self-rotation maximum in the section with = 180Degrees. This pseudo-crystallographic symmetry element was also found to be the twinning axis of pseudo-merohedrally twinned crystals, leading to apparent pseudo-tetragonal P4212 crystal symmetry. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section D: Biological Crystallography International Union of Crystallography

Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally twinned crystals of rubredoxin oxygen oxidoreductase from Desulfovibrio gigas

Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally twinned crystals of rubredoxin oxygen oxidoreductase from Desulfovibrio gigas

Acta Crystallographica Section D: Biological Crystallography , Volume 55 (8): 1465 – Aug 1, 1999

Abstract

Crystals of rubredoxin oxygen oxidoreductase have been obtained and characterized. They belong to space group P21212, with unit-cell dimensions a = 88.24 (15), b = 101.25 (7), c = 90.80 (3) A. The homodimer (86 kDa) in the asymmetric unit is related by a non-crystallographic twofold rotation axis parallel to the ab `diagonal' direction, as shown by the self-rotation maximum in the section with = 180Degrees. This pseudo-crystallographic symmetry element was also found to be the twinning axis of pseudo-merohedrally twinned crystals, leading to apparent pseudo-tetragonal P4212 crystal symmetry.

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References (21)

Publisher
International Union of Crystallography
Copyright
Copyright (c) 1999 International Union of Crystallography
Subject
oxidoreductases, twinning, rubredoxin oxygen oxidoreductase
ISSN
0907-4449
eISSN
1399-0047
DOI
10.1107/S0907444999006216
Publisher site
See Article on Publisher Site

Abstract

Crystals of rubredoxin oxygen oxidoreductase have been obtained and characterized. They belong to space group P21212, with unit-cell dimensions a = 88.24 (15), b = 101.25 (7), c = 90.80 (3) A. The homodimer (86 kDa) in the asymmetric unit is related by a non-crystallographic twofold rotation axis parallel to the ab `diagonal' direction, as shown by the self-rotation maximum in the section with = 180Degrees. This pseudo-crystallographic symmetry element was also found to be the twinning axis of pseudo-merohedrally twinned crystals, leading to apparent pseudo-tetragonal P4212 crystal symmetry.

Journal

Acta Crystallographica Section D: Biological CrystallographyInternational Union of Crystallography

Published: Aug 1, 1999

Keywords: oxidoreductases; twinning; rubredoxin oxygen oxidoreductase.

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