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AbstractThioflavin- T (ThT) is the most commonly used fluorescent dye for following amyloid formation semi-quantitatively in vitro, specifically probing the fibrillar cross-β- sheet content. In recent ...
the amyloid ‐fibrillation condition. From the analyses of CD spectra and thioflavine T fluorescences, it was suggested that variation in residual structure led to different amyloid formation. Interestingly ...
as representative aromatic amino acids of tryptophan or tyrosine were found to greatly suppress the aggregation as evaluated by thioflavin T assay. Circular dichroism spectra showed that the β‐ sheet formation of Aβ42 ...
, we explored the structure -activity relation - ship of naturally-occurring biflavonoids on Aβ amyloidogenesis utilizing an in vitro thioflavin T assay with Aβ1-42 peptide which is prone to aggregate ...
Different low molecular mass ligands have been used to identify amyloid deposits. Among these markers, the dyes Thioflavin T and Congo Red interact specifically with the β‐ sheet structure arranged ...
are much smaller than fibrils. FOs are soluble at 100,000 × g, rich in β- sheet structures , but yet bind weakly to thioflavin T . EPR spectroscopy indicates that FOs display significantly more spin-spin ...
Positron emission tomography (PET) tracer molecules like thioflavin T specifically recognize amyloid deposition in brain tissue by selective binding to hydrophobic or aromatic surface grooves ...
and only exists as a random coil owing to charge repulsion, peptide 1 forms a stable β‐ sheet at neutral pH in aqueous medium. The weakly basic GCP groups (pKa≈7) are key for secondary structure formation ...
attributable to internalization of hydrophobic clusters owing to protein‐protein interaction . Circular dichroism and FTIR spectroscopy further revealed the existence of β sheet structure ...
thioflavin T to reveal the presence of fibrils. FTIR quantitative analysis evidenced a prevalent random conformation at t = 0 with and without Hyp. Fibrils showed a predominant parallel β- sheet structure ...
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