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solution. This protocol is defined as minimum volume cooling (MVC) vitrification . Various types of cryodevices, such as open pulled straw, Cryoloop , and Cryotop, have been developed to accelerate the cooling ...
Cryopreservation/Cryobiology 81 IN VITRO MATURATION , FERTILIZATION AND DEVELOPMENT OF BOVINE IMMATURE OOCYTES CRYOPRESERVED BY VITRIFICATION WITH STEPWISE EXPOSURE USING A NYLON MESH Y. AbeA , K. HaraA , H ...
% survived and 6% hatched. Transfer into three recipients, each with two embryos vitrified with Procedure B, resulted in a successful twin-term pregnancy. CONCLUSION: Modified cryoloop vitrification ...
following vitrification by the PFSV method Reproduction, Fertility and Development Cryopreservation/Cryobiology ABSTRACTS FOR POSTER PRESENTATION Cryopreservation/Cryobiology 81 IN VITRO MATURATION ...
collected and then matured in vitro without vitrification . After IVM, the fresh or vitrified oocytes were used for IVF as follows. IVF and sperm cryopreservation IVF was performed as described by Kohaya et al ...
mm also contain oocytes that are capable of maturation in vitro , but they are much less successful , probably because of cytoplasmic immaturity (25,26). The numbers of oocytes that are recovered ...
in cryopreservation solutions on subsequent embryo development in culture Two-cell embryos that were cryopreserved using either slow-freezing or vitrification procedures developed to the blastocyst stage at high rates ...
vitrification on MI, prophase I (immature) oocytes were vitrified and thawed followed by in vitro meiotic maturation ; controls were isolated and matured without freeze-thaw. Vitrification of oocytes at prophase I ...
., Machida, T. and Kasai, M. ( 1993) Successful vitrification of bovine blastocysts, derived by in vitro maturation and fertilization. Mol. Reprod. Dev. , 34, 266–271. Google Scholar Valdez, C., Albas-Mazni, O ...
for vitrification increases the cooling rate and allows successful cryopreservation of bovine oocytes. Theriogenology , 53, 248 (abstract). Google Scholar Ashwood-Smith, M.J. and Simons, R. ( 1986) The freezing ...
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