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nuclei after a whole-mount FISH preparation. 45S rDNA has been visualized via FISH using a specific probe (red), and DNA has been stained with DAPI (cyan). Dashed circles highlight the nucleolus . Scale ...
not been reported. This transcription was investigated in the present study by simultaneous visualization of the rRNA genes and the rRNA by fluorescent in situ hybridization using a porcine 28S rDNA probe ...
transcribed active genes per individual nucleolus . Visualization of actively transcribed gene clusters is approached by an integrated experimental assay using video microscopy, confocal laser scan microscopy ...
another, but they remain in close proximity for some duration. This approach allows us to detect, track and label transient gene communities ( clusters ) in the nucleolus . Simultaneously , we record summary ...
. Transcription by Pol I but also maturation steps of the precursor are performed in the nucleolus . There is now extensive evidence of 5S gene clusters at chromosomal locations distant from the 45S rDNA ...
. However, the simultaneous visualization of nucleoli and NORs reveals the participation of multiple NORs in the formation of a single nucleolus . The FISH images directly demonstrate the functional redundancy ...
genome contains approximately 400 identical copies of rDNA sequences which are clustered together in head-to-tail arrays at the well known chromosome positions.1 While many rDNA clusters ...
functioning in ribosome biogenesis and acting as a stress sensor. The nucleolus is formed around transcribed ribosomal RNA genes ( rDNA ), with each cell containing hundreds of ribosomal RNA genes, distributed ...
of interphase nuclei where the ribosomal DNA ( rDNA ) genes of all the chromosomes are clustered and the ribosome assembly takes place ( 1 ). In higher eukaryotic cells, the nucleolus lies at or near the nuclear ...
the functional relevance of homotypic clustering of transcription factor binding sites, we studied the 3′-terminal region of murine rRNA genes ( rDNA ), which contains ten binding sites (T1–T10 ...
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