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animal skin. Medical Mycology February 2005, 43,21/26 DNA sequence diversity of intergenic spacer 1 region in the non - lipid -dependent species Malassezia pachydermatis isolated from animals T. SUGITA*, K ...
employed a DNA barcoding approach, targeting conventional and non -conventional genome regions . Genomic analyses were performed using two internal transcribed spacers (nuclear ITS1 and ITS2 sequences ...
of non -coding DNA . The size expansion is due to two very long intergenic spacers (LIGSs), rich in tandem repeats. The two LIGSs are present in all species but vary greatly in length (114–10,408 bp), show ...
in the first helix and loop region of the DNA binding domain We showed above that the purified DNA binding domain of the CaHms1 protein exhibits a strong preference for its cognate DNA binding sequence (a non ...
dogs and cats. Med Mycol . 2001; 39: 329– 334. Google Scholar CrossRef Search ADS PubMed 9. Sugita T, Takeo K, Hama K et al. DNA sequence diversity of intergenic spacer I region in the non - lipid ...
another, or to the spacer region in the S.pombe protein. Sequences within these non -zinc finger regions , however, have been shown to be necessary for support of 5S rRNA synthesis in both the X.laevis ( 21 ...
one copy of the 3XHA tag, which is serving as spacer DNA . Cells were grown at 30° in YPD medium until cultures reached a density of 1 –2 × 107 cells per ml for isolation of either RNA or chromatin ...
was incubated for 1 h at 60°C. The extract was transferred to a phase lock gel tube (prime5, Fisher Scientific SL, Madrid, Spain) for a phenol/chloroform DNA extraction. Genome sequencing Genomic DNA (2–5 μg ...
analysis, a highly conserved PAM for Cas9Smu was not evident. Rather, a significant number of S. mutans strains with Cas9 contained spacers that might require the 5’-NGG-3’ recognition sequence for phage DNA ...
as the hot spot of variation in Antarctic fish mitochondrial genomes. Analyzing the sequence of several intergenic spacers and mapping the arrangements on a newly generated phylogeny showed that the entire ...
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