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. In the present study, we describe a novel approach based on immunization with either genetically modified tumor cells or naked DNA vectors encoding TAE fused to an endoplasmic reticulum (ER) signal sequence (ER ...
to untreated T cells . Thirdly, monocyte derived DCs electrofused with B-CLL B cells were compared with lysate-pulsed DCs . T cells stimulated by fused DCs generated higher levels of specific cytotoxicity ...
analysis . Analysis of In Vivo Priming of Antigen-Specific T- Cells by iPS- DCs Transfectant or nontransfectant iPS- DCs were stimulated with IL-4, TNF-α, and anti-CD40 mAb and injected intraperitoneally ...
-stimulating factor; mES- DC , mature embryonic stem cell -derived dendritic cells ; TNF-α, tumor necrosis factor α. Flow Cytometric Analysis The following monoclonal antibodies (Ab) conjugated with fluorescein ...
expressed 4-1BB constitutively, which was down-regulated by anti-CD40 stimulation. Co-culture of splenic DC with 4-1BBL- transfected cells or 4-1BBL-expressing tumor cell lines led to cytokine (IL-6 and IL-12 ...
experiments. a: P < 0.01 compared with cells without BSA–AGEs, b: P < 0.05 compared with cells incubated with BSA–AGEs and with DC or PAO. (B) Luciferase activity of cells transfected with pGL3-RAGE. A scheme ...
assembled downstream of the GFP coding region, as shown. B, Example of FACS analysis with GFP-A5- transfected cells . Histogram showing the fluorescence of cells transfected with 20 μg of plasmid GFP-A5 ...
production by splenic T cells . T.G activated DCs caused a led to significant elevation of Tcell proliferation. T.G activated DC vaccination resulted in tumor size reduction comparing to controls. T.G lysate ...
in the luciferase activity compared to mock transfected cells (Fig. 2L). Furthermore, western blot analysis was performed in order to confirm the effect of both miRNAs on K-Ras/Erk1/2 and PI3K/Akt signaling pathways ...
) to examine the effect of wt-Ras and RasV12 expression. Of these neuroblastoma cell lines, we could achieve Ras expression levels comparable to those expressed in tumor samples on immunoblot analysis in SH-SY5Y ...
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