Access the full text.
Sign up today, get unlimited access with DeepDyve Pro!
The activity of penicillin acylase has been studied in aqueous and organic solvents, as free enzyme as well as immobilized within the membrane of liquid‐core capsules. The activity of the enzyme ...
as the carrier material for the preparation of monolithic biocatalysts. This protocol leads to active immobilized biocatalysts for the enzymatic hydrolysis of penicillin G (PenG). The monolithic biocatalyst ...
. Immobilization of penicillin G acylase The PGA solution was diluted 45 times with PBS (pH = 8). The process of immobilization of PGA was shown in Fig 1. The 0.1 g magnetic Ni0.4Cu0.5Zn0.1Fe2O4@SiO2-GA ...
blot. Penicillin G acylase activity The enzyme activity of PdPGA was determined by allowing the purified enzyme preparation (0.04 mg/ml) to act on its substrate penicillin G (20 mg/ml) in 50 mM phosphate ...
cephalosporin G by immobilized penicillin G acylase Biochem Eng J 2005 23 203 210 10.1016/j.bej.2005.01.017 Google Scholar Crossref Search ADS WorldCat 19. Patett F , Fischer L Spectrophotometric assay ...
by confocal scanning microscopy. Polyhydroxyoctanoate could not be hydrolyzed by this novel immobilized biocatalyst, whereas the attached enzyme was active in the hydrolysis of p-nitrophenyl alkanoate esters ...
= 318.62 U/ g . The operational stability of immobilized CRL was tested repeatedly, and after 12 cycles of reuse, the enzyme retained 50% activity . Based on our results, we propose that PHBV- immobilized CRL ...
, negative control requires the removal of repressor proteins in order to activate the genes encoding secondary metabolite biosynthesis [7]. Classic UV or chemical mutagenetic programs for strain improvement ...
treated cells with CTAB and the penicillin acylase activity increased about twofold [24]. Therefore, we investigated the use of CTAB as a potential detergent to permeabilize the whole cells of Alcaligenes ...
Search ADS PubMed WorldCat 23. Xue YP , Jiang T, Liu X, Zheng YG Efficient production of S-(+)-2-chlorophenylglycine by immobilized penicillin G acylase in a recirculating packed bed reactor Biochem Eng J ...
Access the full text.
Sign up today, get unlimited access with DeepDyve Pro!