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in Mueller–Hinton medium (McF turbidity of 0.5 each). Here cover slips were used instead of metal discs to allow the microscopy of the biofilms by fluorescence light microscopy. The discs were incubated for 72 ...
with a thin layer of topical triple antibiotic ointment (Bacitracin zinc 400 units , neomycin 3.5 mg, polymyxin B sulfate 5000 units , Actavase, Lincolnton, NC). The animals were covered with nonadherent gauze ...
isothiocyanate–conjugated polyclonal goat antiserum reactive with human IgG heavy and light chains diluted 1:100 in PBS with eriochrome black counterstain. After 30 minutes in a moist chamber at room temperature ...
, motor proteins cause microtubules to glide in motility assays in vitro (Vale et al., 1985). The motor activity of ATP-MAPs was determined by gliding assays of microtubules on ATP-MAPs bound to cover slips ...
. Subsequently, we transferred spermatocyte cysts to poly-D-lysine-coated cover glass dishes and performed live imaging using Andor spinning disk confocal microscopy as described in the methods section . We chose ...
of x, y, and z optical 0.45-μm sections , respectively. (A) Global view of the population of WLIM1-GFP–expressing cells. Bar = 50 μm. (B) Enlarged view of a cell showing transcytoplasmic fluorescent ...
(Diaminobenzidine, Vector) at RT. Slides were counterstained with hematoxylin and dehydrated for cover slipping . Histopathology quantification was performed for blood vessels, epithelial thickness, and collagen ...
; see Supplementary Material, Methods and Fig. S1) and assessed as possibly damaging by multiple algorithms (data not shown). Accordingly, we revised our patients’ diagnosis to WS, pending confirmation ...
. The slides were incubated in a moist chamber at room temperature for 1 hr or overnight. In the latter case, the slides were sealed with rubber cement. Cover slips were removed, and the slides were washed twice ...
to inhibit KCSs through the covalent modification of the active site (Boger et al., 2000; Trenkamp et al., 2004). Rescue of trichome development became apparent within several days after multiple applications ...
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