Application of RAPD markers in fish: Part I – some genera (Tilapia, Sarotherodon and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia

Application of RAPD markers in fish: Part I – some genera (Tilapia, Sarotherodon and... Random amplified polymorphic DNA (RAPD) analysis was applied to the four-tilapia species (three genera): redbelly tilapia (Tilapia zillii), white tilapia (Sarotherodon galilaeus), blue tilapia (Oreochromis aureus) and Nile tilapia (Oreochromis niloticus). Twenty random primers – fifteen (10-mer) and five (20-mer) – were used to assay polymorphisms among three genera and between two species. Different RAPD fragment patterns were observed for different genera species. Results showed that there are great differences among the three genera of tilapia fish. Also, data demonstrated that RAPD markers are useful for the systematic investigation at the level of tilapia species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Biotechnology Inderscience Publishers

Application of RAPD markers in fish: Part I – some genera (Tilapia, Sarotherodon and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia

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Publisher
Inderscience Publishers
Copyright
Copyright © Inderscience Enterprises Ltd. All rights reserved
ISSN
0963-6048
eISSN
1741-5020
Publisher site
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Abstract

Random amplified polymorphic DNA (RAPD) analysis was applied to the four-tilapia species (three genera): redbelly tilapia (Tilapia zillii), white tilapia (Sarotherodon galilaeus), blue tilapia (Oreochromis aureus) and Nile tilapia (Oreochromis niloticus). Twenty random primers – fifteen (10-mer) and five (20-mer) – were used to assay polymorphisms among three genera and between two species. Different RAPD fragment patterns were observed for different genera species. Results showed that there are great differences among the three genera of tilapia fish. Also, data demonstrated that RAPD markers are useful for the systematic investigation at the level of tilapia species.

Journal

International Journal of BiotechnologyInderscience Publishers

Published: Jan 1, 2004

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