Departmt of Biochemistry and Biophysics, University of Calqornia, Sun Francisco, California 94143 Manuscript received September 22, 1995 Accepted for publication December 13, 1995 ABSTRACT We isolated and characterized numerous grailed and inuected alleles. Among the deficicies w e isolated, a mutant lacking invected sequces wasviable and photypically normal, a mutant lacking grailed was an embryo lethal and had slight segmtation defects, andmutant lacking both grailed a and inuectedwas most severely affected. In sev grailed alleles, mutations caused translation to terminate prematurely in the ctral or Gterminal portionthe coding sequce, resulting in embryonic lethality of and segmtation defects. Bothgrailed and invected expression declined prematurely in these mutant embryos. In wild-type embryos, grailed and invected are juxtaposed and are expressed in esstially mgrailed and invected transcription units idtical patterns. A breakpoint mutant that separates the parceled differt aspects of the expression pattern to grailed or inuected. W also found that both e ges cause similar defects wh expressed ectopically and the protein productsof both ges act that e to repress transcription in cultured cells.W propose that the varied photypes of the grailed alleles can be explained b the differtial effects these mutants have on the combination y of grailed and
Genetics – Genetics Society of America
Published: Mar 1, 1996
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