Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the Fok I endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites. Footnotes Supporting information is available online at http://www.genetics.org/cgi/content/full/genetics.110.120717/DC1 . Communicating editor: J. B oeke Received July 8, 2010. Accepted July 20, 2010. Copyright © 2010 by the Genetics Society of America Available freely online through the author-supported open access option. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

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Publisher
Genetics Society of America
Copyright
Copyright © 2010 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
DOI
10.1534/genetics.110.120717
Publisher site
See Article on Publisher Site

Abstract

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the Fok I endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites. Footnotes Supporting information is available online at http://www.genetics.org/cgi/content/full/genetics.110.120717/DC1 . Communicating editor: J. B oeke Received July 8, 2010. Accepted July 20, 2010. Copyright © 2010 by the Genetics Society of America Available freely online through the author-supported open access option.

Journal

GeneticsGenetics Society of America

Published: Oct 1, 2010

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