Large Scale Identification of Genes Involved in Cell Surface Biosynthesis and Architecture in Saccharomyces cerevisiae

Large Scale Identification of Genes Involved in Cell Surface Biosynthesis and Architecture in... M. Lussier, A. M. White, J. Sheraton, T. di-Paolo, J. Treadwell, S. B. Southard, C. I. Horenstein, J. Chen-Weiner, AFJ. Ram, J. C. Kapteyn, T. W. Roemer, D. H. Vo, D. C. Bondoc, J. Hall, W. Wei Zhong, A. M. Sdicu, J. Davies, F. M. Klis, P. W. Robbins and H. Bussey Department of Biology, McGill University, Montreal, Quebec, Canada The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

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Publisher
Genetics Society of America
Copyright
Copyright © 1997 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
Publisher site
See Article on Publisher Site

Abstract

M. Lussier, A. M. White, J. Sheraton, T. di-Paolo, J. Treadwell, S. B. Southard, C. I. Horenstein, J. Chen-Weiner, AFJ. Ram, J. C. Kapteyn, T. W. Roemer, D. H. Vo, D. C. Bondoc, J. Hall, W. Wei Zhong, A. M. Sdicu, J. Davies, F. M. Klis, P. W. Robbins and H. Bussey Department of Biology, McGill University, Montreal, Quebec, Canada The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.

Journal

GeneticsGenetics Society of America

Published: Oct 1, 1997

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