Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive Expression of ATL2, an Early Elicitor-Response RING-H2 Zinc-Finger Gene

Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive... Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of pathogen response. We screened 50,000 M 2 families from a line that carries a fusion of p ATL2 to the GUS reporter gene and isolated five mutants, which we named eca ( e xpresión c onstitutiva de A TL2), that showed constitutive expression of the reporter gene. One mutant exhibits a drastic stunted phenotype while the other four grow similarly to wild type. Two early chitin-induced genes and known pathogenesis-related genes such as NPR1 , PAL , and CHS are activated in all the mutants whereas members of the ATL family and PR-1 and PDF2.1 , which are markers of the salicylic acid (SA) jasmonate (JA) defense-response pathways, display differential expression between the mutants. These observations indicate that the ECA gene products may function in the early steps of an elicitor-response pathway, although some of them may function at other stages on the SA or JA defense-response pathways. Likewise, the fact that ATL2 and other members of the ATL family are activated in eca mutants links the induction of this putative class of ubiquitin ligases to plant defense signaling pathways. Footnotes This article is dedicated to the memory of the late Gilberto Mosqueda Cano. Communicating editor: D. W eigel Received February 24, 2004. Accepted March 3, 2004. Genetics Society of America http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive Expression of ATL2, an Early Elicitor-Response RING-H2 Zinc-Finger Gene

Genetics, Volume 167 (2): 919 – Jun 1, 2004

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Publisher
Genetics Society of America
Copyright
Copyright © 2004 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
DOI
10.1534/genetics.104.028043
Publisher site
See Article on Publisher Site

Abstract

Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of pathogen response. We screened 50,000 M 2 families from a line that carries a fusion of p ATL2 to the GUS reporter gene and isolated five mutants, which we named eca ( e xpresión c onstitutiva de A TL2), that showed constitutive expression of the reporter gene. One mutant exhibits a drastic stunted phenotype while the other four grow similarly to wild type. Two early chitin-induced genes and known pathogenesis-related genes such as NPR1 , PAL , and CHS are activated in all the mutants whereas members of the ATL family and PR-1 and PDF2.1 , which are markers of the salicylic acid (SA) jasmonate (JA) defense-response pathways, display differential expression between the mutants. These observations indicate that the ECA gene products may function in the early steps of an elicitor-response pathway, although some of them may function at other stages on the SA or JA defense-response pathways. Likewise, the fact that ATL2 and other members of the ATL family are activated in eca mutants links the induction of this putative class of ubiquitin ligases to plant defense signaling pathways. Footnotes This article is dedicated to the memory of the late Gilberto Mosqueda Cano. Communicating editor: D. W eigel Received February 24, 2004. Accepted March 3, 2004. Genetics Society of America

Journal

GeneticsGenetics Society of America

Published: Jun 1, 2004

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