Genome Engineering With Zinc-Finger Nucleases

Genome Engineering With Zinc-Finger Nucleases Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been made to genome editing in many different organisms and situations. Considerable progress has been made in methods for deriving zinc-finger sets for new genomic targets, but approaches to design and selection are still being perfected. An issue that needs more attention is the extent to which available mechanisms of double-strand break repair limit the scope and utility of ZFN-initiated events. The bright prospects for future applications of ZFNs, including human gene therapy, are discussed. Copyright © 2011 by the Genetics Society of America « Previous | Next Article » Table of Contents This Article doi: 10.1534/genetics.111.131433 Genetics August 1, 2011 vol. 188 no. 4 773-782 » Abstract Full Text Full Text (PDF) Classifications Review Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Google Scholar Articles by Carroll, D. PubMed PubMed citation Articles by Carroll, D. Related Content Load related web page information This Months's Issue September 2011, 189 (1) Alert me to new issues of Genetics ABOUT SUBSCRIBE INFO FOR AUTHORS ONLINE SUBMISSION EDITORIAL BOARD CONTACT ALERTS HELP ADVERTISING RSS Help Contact Us International Access Link Print ISSN: 0016-6731 Online ISSN: 1943-2631 Copyright © 2011 by the Genetics Society of America phone: 412-268-1812 fax: 412-268-1813 email: genetics-gsa@andrew.cmu.edu http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

Genome Engineering With Zinc-Finger Nucleases

Genetics, Volume 188 (4): 773 – Aug 1, 2011

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Publisher
Genetics Society of America
Copyright
Copyright © 2011 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
D.O.I.
10.1534/genetics.111.131433
Publisher site
See Article on Publisher Site

Abstract

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been made to genome editing in many different organisms and situations. Considerable progress has been made in methods for deriving zinc-finger sets for new genomic targets, but approaches to design and selection are still being perfected. An issue that needs more attention is the extent to which available mechanisms of double-strand break repair limit the scope and utility of ZFN-initiated events. The bright prospects for future applications of ZFNs, including human gene therapy, are discussed. Copyright © 2011 by the Genetics Society of America « Previous | Next Article » Table of Contents This Article doi: 10.1534/genetics.111.131433 Genetics August 1, 2011 vol. 188 no. 4 773-782 » Abstract Full Text Full Text (PDF) Classifications Review Services Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Citing Articles Load citing article information Google Scholar Articles by Carroll, D. PubMed PubMed citation Articles by Carroll, D. Related Content Load related web page information This Months's Issue September 2011, 189 (1) Alert me to new issues of Genetics ABOUT SUBSCRIBE INFO FOR AUTHORS ONLINE SUBMISSION EDITORIAL BOARD CONTACT ALERTS HELP ADVERTISING RSS Help Contact Us International Access Link Print ISSN: 0016-6731 Online ISSN: 1943-2631 Copyright © 2011 by the Genetics Society of America phone: 412-268-1812 fax: 412-268-1813 email: genetics-gsa@andrew.cmu.edu

Journal

GeneticsGenetics Society of America

Published: Aug 1, 2011

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