Víctor Quesada a , María Rosa Ponce a , and José Luis Micol a a División de Genética, Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain Corresponding author: José Luis Micol, División de Genética, Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain., firstname.lastname@example.org (E-mail) Communicating editor: V. S UNDARESAN Stress caused by the increased salinity of irrigated fields impairs plant growth and is one of the major constraints that limits crop productivity in many important agricultural areas. As a contribution to solving such agronomic problems, we have carried out a large-scale screening for Arabidopsis thaliana mutants induced on different genetic backgrounds by EMS treatment, fast neutron bombardment, or T-DNA insertions. From the 675,500 seeds we screened, 17 mutant lines were isolated, all but one of which yielded 25–70% germination levels on 250 m M NaCl medium, a condition in which their ancestor ecotypes are unable to germinate. Monogenic recessive inheritance of NaCl-tolerant germination was displayed with incomplete penetrance by all the selected mutants, which fell into five complementation groups. These were named SALOBREÑO ( SAÑ ) and mapped relative to polymorphic microsatellites, the map positions of three of them suggesting that they are novel genes. Strains carrying mutations in the SAÑ1 - SAÑ4 genes display similar responses to both ionic effects and osmotic pressure, their germination being NaCl and mannitol tolerant but KCl and Na 2 SO 4 sensitive. In addition, NaCl-, KCl-, and mannitol-tolerant as well as abscisic-acid-insensitive germination was displayed by sañ5 , whose genetic and molecular characterization indicates that it carries an extremely hypomorphic or null allele of the ABI4 gene, its deduced protein product lacking the APETALA2 DNA binding domain.
Genetics – Genetics Society of America
Published: Jan 1, 2000
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