FRIGIDA ( FRI ) and FLOWERING LOCUS C ( FLC ) are two genes that, unless plants are vernalized, greatly delay flowering time in Arabidopsis thaliana . Natural loss-of-function mutations in FRI cause the early flowering growth habits of many A. thaliana accessions. To quantify the variation among wild accessions due to FRI , and to identify additional genetic loci in wild accessions that influence flowering time, we surveyed the flowering times of 145 accessions in long-day photoperiods, with and without a 30-day vernalization treatment, and genotyped them for two common natural lesions in FRI . FRI is disrupted in at least 84 of the accessions, accounting for only ∼40% of the flowering-time variation in long days. During efforts to dissect the causes for variation that are independent of known dysfunctional FRI alleles, we found new loss-of-function alleles in FLC , as well as late-flowering alleles that do not map to FRI or FLC . An FLC nonsense mutation was found in the early flowering Van-0 accession, which has otherwise functional FRI . In contrast, Lz-0 flowers late because of high levels of FLC expression, even though it has a deletion in FRI . Finally, eXtreme array mapping identified genomic regions linked to the vernalization-independent, late-flowering habit of Bur-0, which has an alternatively spliced FLC allele that behaves as a null allele. Footnotes ↵ 1 Present address: EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. Sequences data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. AY781906 , AY785055 , AY849982 , AY849983 , AY849984 , AY849985 , AY849986 , AY849987 , AY849988 , AY849989 , AY849990 , AY849991 , AY849992 , AY849993 , AY849994 , AY849995 , AY849996 , AY849997 , AY849998 , AY849999 , AY850000 , AY850001 , AY850002 , AY854371 , AY854372 , and AH014566 . Communicating editor: V. S undaresan Received September 20, 2004. Accepted January 3, 2005. Genetics Society of America
Genetics – Genetics Society of America
Published: Jul 1, 2005
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera