EMS-Induced Polygenic Mutation Rates for Nine Quantitative Characters in Drosophila melanogaster

EMS-Induced Polygenic Mutation Rates for Nine Quantitative Characters in Drosophila melanogaster Peter D. Keightley a and Ohmi Ohnishi b a Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, Scotland b Plant Germ-Plasm Institute, Faculty of Agriculture, Kyoto University, Mozume Muko, Kyoto 617, Japan Corresponding author: Peter D. Keightley, Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, p.keightley@edinburgh.ac.uk (E-mail). Communicating editor: M. J. S IMMONS Polygenic mutations were induced by treating Drosophila melanogaster adult males with 2.5 m M EMS. The treated second chromosomes, along with untreated controls, were then made homozygous, and five life history, two behavioral, and two morphological traits were measured. EMS mutagenesis led to reduced performance for life history traits. Changes in means and increments in genetic variance were relatively much higher for life history than for morphological traits, implying large differences in mutational target size. Maximum likelihood was used to estimate mutation rates and parameters of distributions of mutation effects, but parameters were strongly confounded with one another. Several traits showed evidence of leptokurtic distributions of effects and mean effects smaller than a few percent of trait means. Distributions of effects for all traits were strongly asymmetrical, and most mutations were deleterious. Correlations between life history mutation effects were positive. Mutation parameters for one generation of spontaneous mutation were predicted by scaling parameter estimates from the EMS experiment, extrapolated to the whole genome. Predicted mutational coefficients of variation were in good agreement with published estimates. Predicted changes in means were up to 0.14% or 0.6% for life history traits, depending on the model of scaling assumed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

EMS-Induced Polygenic Mutation Rates for Nine Quantitative Characters in Drosophila melanogaster

Genetics, Volume 148 (2): 753 – Feb 1, 1998

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Publisher
Genetics Society of America
Copyright
Copyright © 1998 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
Publisher site
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Abstract

Peter D. Keightley a and Ohmi Ohnishi b a Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, Scotland b Plant Germ-Plasm Institute, Faculty of Agriculture, Kyoto University, Mozume Muko, Kyoto 617, Japan Corresponding author: Peter D. Keightley, Institute of Cell, Animal and Population Biology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, Scotland, p.keightley@edinburgh.ac.uk (E-mail). Communicating editor: M. J. S IMMONS Polygenic mutations were induced by treating Drosophila melanogaster adult males with 2.5 m M EMS. The treated second chromosomes, along with untreated controls, were then made homozygous, and five life history, two behavioral, and two morphological traits were measured. EMS mutagenesis led to reduced performance for life history traits. Changes in means and increments in genetic variance were relatively much higher for life history than for morphological traits, implying large differences in mutational target size. Maximum likelihood was used to estimate mutation rates and parameters of distributions of mutation effects, but parameters were strongly confounded with one another. Several traits showed evidence of leptokurtic distributions of effects and mean effects smaller than a few percent of trait means. Distributions of effects for all traits were strongly asymmetrical, and most mutations were deleterious. Correlations between life history mutation effects were positive. Mutation parameters for one generation of spontaneous mutation were predicted by scaling parameter estimates from the EMS experiment, extrapolated to the whole genome. Predicted mutational coefficients of variation were in good agreement with published estimates. Predicted changes in means were up to 0.14% or 0.6% for life history traits, depending on the model of scaling assumed.

Journal

GeneticsGenetics Society of America

Published: Feb 1, 1998

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