Ac Transposition From a T-DNA Can Generate Linked and Unlinked Clusters of Insertions in the Tomato Genome

Ac Transposition From a T-DNA Can Generate Linked and Unlinked Clusters of Insertions in the... B. I. Osborne, C. A. Corr, J. P. Prince, R. Hehl, S. D. Tanksley, S. McCormick and B. Baker Plant Gene Expression Center, Albany, California, 94710, Department of Plant Pathology, University of California, Berkeley, California 94720 We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

Ac Transposition From a T-DNA Can Generate Linked and Unlinked Clusters of Insertions in the Tomato Genome

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Publisher
Genetics Society of America
Copyright
Copyright © 1991 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
Publisher site
See Article on Publisher Site

Abstract

B. I. Osborne, C. A. Corr, J. P. Prince, R. Hehl, S. D. Tanksley, S. McCormick and B. Baker Plant Gene Expression Center, Albany, California, 94710, Department of Plant Pathology, University of California, Berkeley, California 94720 We have investigated the distribution of transposed Acs in the tomato genome. Our approach has been to clone the regions flanking the T-DNAs and transposed Acs from two transgenic lines of tomato and place these sequences on the tomato restriction fragment length polymorphism (RFLP) map. The distribution of transposed Acs around the T-DNA and at locations unlinked to the T-DNA indicates that Ac transposes to linked and unlinked sites in tomato as it does in maize. The structure and terminal sequence of these cloned elements shows that Ac remains intact after transposition. We discuss these results and their bearing on gene tagging strategies using Ac and Ds.

Journal

GeneticsGenetics Society of America

Published: Nov 1, 1991

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