A Stable Genomic Source of P Element Transposase in Drosophila melanogaster

A Stable Genomic Source of P Element Transposase in Drosophila melanogaster H. M. Robertson, C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz and W. R. Engels Present address: Department of Entomology, University of Illinois, Urbana, Illinois 61801. A single P element insert in Drosophila melanogaster, called P(ry(+){Delta}2-3)(99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for ``helper'' plasmids in P element mediated transformation, and can be used to move transformed elements around the genome. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

A Stable Genomic Source of P Element Transposase in Drosophila melanogaster

Loading next page...
 
/lp/genetics-society-of-america/a-stable-genomic-source-of-p-element-transposase-in-drosophila-hCWUIi04vq
Publisher site
See Article on Publisher Site

Abstract

H. M. Robertson, C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz and W. R. Engels Present address: Department of Entomology, University of Illinois, Urbana, Illinois 61801. A single P element insert in Drosophila melanogaster, called P(ry(+){Delta}2-3)(99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for ``helper'' plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.

Journal

GeneticsGenetics Society of America

Published: Mar 1, 1988

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off