A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana Eiji Nambara a,b , Masaharu Suzuki c , Suzanne Abrams d , Donald R. McCarty c , Yuji Kamiya b , and Peter McCourt a a Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada, b Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Wako, Japan 351-0198, c Horticultural Sciences Department, University of Florida, Gainesville, Florida 32605 d Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada Corresponding author: Peter McCourt, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada., mccourt@botany.utoronto.ca (E-mail) Communicating editor: C. S. G ASSER The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (-)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs . the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2 , and a collection of new mutant alleles of the ABA-insensitive genes, ABI3 , ABI4 , and ABI5 . The abi5 alleles are less sensitive to (+)-ABA than to (-)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

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Publisher
Genetics Society of America
Copyright
Copyright © 2002 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
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Abstract

Eiji Nambara a,b , Masaharu Suzuki c , Suzanne Abrams d , Donald R. McCarty c , Yuji Kamiya b , and Peter McCourt a a Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada, b Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Wako, Japan 351-0198, c Horticultural Sciences Department, University of Florida, Gainesville, Florida 32605 d Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada Corresponding author: Peter McCourt, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada., mccourt@botany.utoronto.ca (E-mail) Communicating editor: C. S. G ASSER The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (-)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs . the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2 , and a collection of new mutant alleles of the ABA-insensitive genes, ABI3 , ABI4 , and ABI5 . The abi5 alleles are less sensitive to (+)-ABA than to (-)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals.

Journal

GeneticsGenetics Society of America

Published: Jul 1, 2002

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