A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus

A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus Geoffrey C. Waldbieser a , Brian G. Bosworth a , Danny J. Nonneman a , and William R. Wolters a a U.S. Department of Agriculture-Agricultural Research Service, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, Mississippi 38776 Corresponding author: Geoffrey C. Waldbieser, USDA-ARS, Catfish Genetics Research Unit, P.O. Box 38, Stoneville, MS 38776., gwaldbieser@ars.usda.gov (E-mail) Communicating editor: G. B. G OLDING Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome ( N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the efficiency of selective breeding programs for channel catfish. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genetics Genetics Society of America

A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus

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Publisher
Genetics Society of America
Copyright
Copyright © 2001 by the Genetics Society of America
ISSN
0016-6731
eISSN
1943-2631
Publisher site
See Article on Publisher Site

Abstract

Geoffrey C. Waldbieser a , Brian G. Bosworth a , Danny J. Nonneman a , and William R. Wolters a a U.S. Department of Agriculture-Agricultural Research Service, Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, Mississippi 38776 Corresponding author: Geoffrey C. Waldbieser, USDA-ARS, Catfish Genetics Research Unit, P.O. Box 38, Stoneville, MS 38776., gwaldbieser@ars.usda.gov (E-mail) Communicating editor: G. B. G OLDING Microsatellite loci were identified in channel catfish gene sequences or random clones from a small insert genomic DNA library. Outbred populations of channel catfish contained an average of eight alleles per locus and an average heterozygosity of 0.70. A genetic linkage map of the channel catfish genome ( N = 29) was constructed from two reference families. A total of 293 microsatellite loci were polymorphic in one or both families, with an average of 171 informative meioses per locus. Nineteen type I loci, 243 type II loci, and one EST were placed in 32 multipoint linkage groups covering 1958 cM. Nine more type II loci were contained in three two-point linkage groups covering 24.5 cM. Twenty-two type II loci remained unlinked. Multipoint linkage groups ranged in size from 11.9 to 110.5 cM with an average intermarker distance of 8.7 cM. Seven microsatellite loci were closely linked with the sex-determining locus. The microsatellite loci and genetic linkage map will increase the efficiency of selective breeding programs for channel catfish.

Journal

GeneticsGenetics Society of America

Published: Jun 1, 2001

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