Optimizing β-carotene production by Blakeslea trispora using bug damaged wheat

Optimizing β-carotene production by Blakeslea trispora using bug damaged wheat Purposeβ-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of β-carotene by Blakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source.Design/methodology/approachResponse surface methodology (RSM) has been used to determine the optimum concentrations of media components for the maximum β-carotene production. β-carotene concentration was determined quantitively using a spectrophotometric method.FindingsA full quadratic model fit the data best for β-carotene production with a high R2 value (0.999). Using optimized media increased the production of β-carotene by B. trispora DSM-2387 and B. trispora DSM-2388 to a higher level (250.407 mg/l) than the control medium (72.195 mg/l).Originality/valueThis study showed that bug-damaged wheat, an agro-industrial waste having low economical value, may be used as a valuable substrate for microbial production of β-carotene, a natural food colourant possessing high economical value. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pigment & Resin Technology Emerald Publishing

Optimizing β-carotene production by Blakeslea trispora using bug damaged wheat

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Publisher
Emerald Group Publishing Limited
Copyright
Copyright © Emerald Group Publishing Limited
ISSN
0369-9420
D.O.I.
10.1108/PRT-05-2017-0055
Publisher site
See Article on Publisher Site

Abstract

Purposeβ-carotene, found naturally in many foods, has been widely used as a colourant in various products. The aim of this study is to optimize the production of β-carotene by Blakeslea trispora using bug-damaged wheat as the main nitrogen and carbon source.Design/methodology/approachResponse surface methodology (RSM) has been used to determine the optimum concentrations of media components for the maximum β-carotene production. β-carotene concentration was determined quantitively using a spectrophotometric method.FindingsA full quadratic model fit the data best for β-carotene production with a high R2 value (0.999). Using optimized media increased the production of β-carotene by B. trispora DSM-2387 and B. trispora DSM-2388 to a higher level (250.407 mg/l) than the control medium (72.195 mg/l).Originality/valueThis study showed that bug-damaged wheat, an agro-industrial waste having low economical value, may be used as a valuable substrate for microbial production of β-carotene, a natural food colourant possessing high economical value.

Journal

Pigment & Resin TechnologyEmerald Publishing

Published: May 8, 2018

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