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Separation of flavonoids in the leaves of Sophora japonica by macroporous adsorption resin mixed-bed technology

Separation of flavonoids in the leaves of Sophora japonica by macroporous adsorption resin... PurposeThe purpose of this paper is to separate and purify flavonoids existing in the leaves of Sophora japonica by a novel method, macroporous adsorption resin (MAR) mixed-bed technology, and the optimal MAR mixed bed was screened based on the adsorption experimental result with the order of single, two, three and four MAR mixed bed separately.Design/methodology/approachThe adsorption performance of MAR and MAR mixed bed for flavonoids was studied using ultraviolet – visible (UV-VIS) spectrophotometry.FindingsThis research showed that the MAR mixed bed of LZ-54 + LZ-67 with a mass ratio of mLZ-54:mLZ-67 = 1:1 was the optimized combination with the optimal conditions of adsorption (volume V = 140 mL, pH = 5, T = 35°C) and desorption (liquid ratio R = 50 per cent, T = 30°C, pH = 6) obtained, relatively.Practical implicationsThis study aims to find an efficient way of separating flavonoids and other components that are useful for human health from Sophora japonica, which is complying with the policy of sustainable development.Originality/valueThis contribution provided a novel way to separate flavonoids from Sophora japonica. Under the optimal conditions, the adsorption rate (F) of MAR mixed bed LZ-54 + LZ-67 to the flavonoids was 63.65 per cent, the desorption rate (D) was 87.31 per cent and the purity was dramatically achieved at 58.17 per cent from 17.67 per cent after a round of adsorption/desorption operation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pigment & Resin Technology Emerald Publishing

Separation of flavonoids in the leaves of Sophora japonica by macroporous adsorption resin mixed-bed technology

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Publisher
Emerald Publishing
Copyright
Copyright © Emerald Group Publishing Limited
ISSN
0369-9420
DOI
10.1108/PRT-01-2016-0007
Publisher site
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Abstract

PurposeThe purpose of this paper is to separate and purify flavonoids existing in the leaves of Sophora japonica by a novel method, macroporous adsorption resin (MAR) mixed-bed technology, and the optimal MAR mixed bed was screened based on the adsorption experimental result with the order of single, two, three and four MAR mixed bed separately.Design/methodology/approachThe adsorption performance of MAR and MAR mixed bed for flavonoids was studied using ultraviolet – visible (UV-VIS) spectrophotometry.FindingsThis research showed that the MAR mixed bed of LZ-54 + LZ-67 with a mass ratio of mLZ-54:mLZ-67 = 1:1 was the optimized combination with the optimal conditions of adsorption (volume V = 140 mL, pH = 5, T = 35°C) and desorption (liquid ratio R = 50 per cent, T = 30°C, pH = 6) obtained, relatively.Practical implicationsThis study aims to find an efficient way of separating flavonoids and other components that are useful for human health from Sophora japonica, which is complying with the policy of sustainable development.Originality/valueThis contribution provided a novel way to separate flavonoids from Sophora japonica. Under the optimal conditions, the adsorption rate (F) of MAR mixed bed LZ-54 + LZ-67 to the flavonoids was 63.65 per cent, the desorption rate (D) was 87.31 per cent and the purity was dramatically achieved at 58.17 per cent from 17.67 per cent after a round of adsorption/desorption operation.

Journal

Pigment & Resin Technology – Emerald Publishing

Published: May 2, 2017

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