Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan Carotenoids are pigments that have significant value as colorants and antioxidants in pharmaceutical, food, textile and cosmetic industries. Owing to its high demand, this study aims to identify ß-carotene producing bacteria from different terrestrial habitats of Jaipur region.Design/methodology/approachIn this paper, standard isolation and purification process was used, followed by colony morphology and biochemical characterization of ß-carotene producing bacteria. ß-carotene concentration was determined quantitatively using spectrophotometric method.FindingsOut of 43 isolates, 21 isolates showed peak range between 400 and 500 nm confirming the presence of carotenoids. Only one bacteria SAN-A has capacity to produce ß-carotene confirmed by the thin layer chromatography and high performance liquid chromatography (HPLC) with a yield of 1.68 mg/l. The 2, 2-Diphenyl-2-picrylhydrazil (DPPH) assay showed an IC50 value of 4.0 mg/ml.Originality/valueThe present study revealed the presence of ß-carotene producing bacteria in the soil of different terrestrial habitat of Jaipur region which can be exploited as an economical source for ß-carotene production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pigment & Resin Technology Emerald Publishing

Identification of ß-carotene producing bacteria from terrestrial habitats of Jaipur, Rajasthan

Download PDF
8 pages

Loading next page...
 
/lp/emerald-publishing/identification-of-carotene-producing-bacteria-from-terrestrial-XXaNpXsgJF
Publisher
Emerald Publishing
Copyright
© Emerald Publishing Limited
ISSN
0369-9420
DOI
10.1108/prt-07-2018-0070
Publisher site
See Article on Publisher Site

Abstract

Carotenoids are pigments that have significant value as colorants and antioxidants in pharmaceutical, food, textile and cosmetic industries. Owing to its high demand, this study aims to identify ß-carotene producing bacteria from different terrestrial habitats of Jaipur region.Design/methodology/approachIn this paper, standard isolation and purification process was used, followed by colony morphology and biochemical characterization of ß-carotene producing bacteria. ß-carotene concentration was determined quantitatively using spectrophotometric method.FindingsOut of 43 isolates, 21 isolates showed peak range between 400 and 500 nm confirming the presence of carotenoids. Only one bacteria SAN-A has capacity to produce ß-carotene confirmed by the thin layer chromatography and high performance liquid chromatography (HPLC) with a yield of 1.68 mg/l. The 2, 2-Diphenyl-2-picrylhydrazil (DPPH) assay showed an IC50 value of 4.0 mg/ml.Originality/valueThe present study revealed the presence of ß-carotene producing bacteria in the soil of different terrestrial habitat of Jaipur region which can be exploited as an economical source for ß-carotene production.

Journal

Pigment & Resin Technology – Emerald Publishing

Published: Jul 5, 2019

Keywords: Antioxidants; Pigments; Bacteria; Carotenoid; HPLC; Jaipur; TLC; Soil; UV-spectrophotometer

References

  • Production of β-carotene by a newly isolated rhodotorula glutinis UCP1555 strain and cytotoxic effect evaluation
    Andrade, R.F.; Lima, R.A.; Ribeaux, D.R.; Araújo, H.W.; Franco, L.O.; Pessoa-Júnior, A.; Campos-Takaki, G.M.
  • Screening of yellow pigment producing bacterial isolates from various eco-climatic areas and analysis of the carotenoid produced by the isolate
    Arulselvi, I.P.; Umamaheswari, S.; Sharma, R.G.; Karthik, C.; Jayakrishna, C.
  • Methods of analysis (extraction, separation, identification and quantification) of carotenoids from natural products
    Butnariu, M.
  • ‘Biotechnological production of carotenoids and their applications in food and pharmaceutical products’
    Cardoso, L.A.C.; Karp, S.G.; Vendruscolo, F.; Kanno, K.Y.F.; Zoz, L.I.C.; Carvalho, J.C.
  • Evaluation of antioxidant properties of a pigment molecule isolated from radioresistan t bacterial strain inm-2
    Chausali, N.; Kumar, R.
  • Simplistic approach in extracellular synthesis of silver nanoparticles via bioreducing potential of Planococcus plakortidis strain BGCC-51 isolated from dye industry effluent soil
    Chokriwal, A.; Stephen, B.J.; Jain, D.; Singh, A.
  • Carotene-oxygen radical interactions
    Conn, P.F.; Lambert, C.; Land, E.J.; Schalch, W.; Truscott, T.G.
  • Thin-layer chromatographic analysis of beta-carotene and lutein in echinostoma trivolvis (trematoda) rediae
    Fried, B.; Beers, K.; Sherma, J.
  • Identification and characterization of extracellular red pigment producing bacteria isolated from soil
    Goswami, B.; Bhowal, J.
  • Haematococcus Astaxanthin: applications for human health and nutrition
    Guerin, M.; Huntley, M.E.; Olaizola, M.
  • Natural useful therapeutic products from microbes
    Gupta, C.; Prakash, D.; Gupta, S.
  • Carotenoid production by Rhodotorula Glutinis YB-252 in solid-state fermentation
    Hernandez-Almanzaa, A.; Montanez-Saenz, J.; Martınez-vila, C.
  • Separation and characterization of antioxidants from Spirulina Platensis microalga combining pressurized liquid extraction, TLC, and HPLC‐DAD
    Jaime, L.; Mendiola, J.A.; Herrero, M.; Soler-Rivas, C.; Santoyo, S.; Señorans, F.J.; Cifuentes, A.; Ibáñez, E.
  • Engineering microbial cell factories for biosynthesis of isoprenoid molecules: beyond lycopene
    Klein-Marcuschamer, D.; Ajikumar, P.K.; Stephanopoulos, G.
  • The biological properties of carotenoids
    Krinsky, N.I.
  • Characterization and cytotoxicity assay of pigment producing microbes
    Malik, K.; Tokas, J.; Anand, R.C.
  • The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease
    Mares-Perlman, J.A.; Millen, A.E.; Ficek, T.L.; Hankinson, S.E.
  • Rapid production of natural carotenoids from Rhodobacter Sphaeroides
    Nie, X.; Xiao, C.; Zhang, L.; Zhao, Y.; Liu, X.; Wang, J.; Tang, Y.; Liu, Y.; Zhao, Z.; Pan, Y.
  • Carotenoid biosynthetic pathway: molecular phylogenies and evolutionary behavior of crt genes in eubacteria
    Phadwal, K.
  • Production of prodigiosin from Serratia Marcescens and its cytotoxicity activity
    Raj, D.N.; Dhanasekaran, D.; Thajuddin, N.; Panneerselvam, A.
  • Isolation & identification of pigment producing bacterial isolates from different terrestrial habitats in thane district, MS, India
    Rajguru, S.A.; Sawant, N.; Valmiki, A.; Deshmukh, P.V.
  • Forming bacterium for carotenogenesis
    Ramasamy, A.K.; Udayasuriyan, V.
  • Carotenoids in crabs from marine and fresh waters of India
    Sachindra, N.M.; Bhaskar, N.; Mahendrakar, N.S.
  • Progress in microbial carotenoids production
    Saini, R.K.; Keum, Y.S.
  • Assay of β-Carotene in dietary supplements and fruit juices by TLC-Densitometry
    Starek, M.; Guja, A.; Dąbrowska, M.; Krzek, J.
  • Bacterial pigments and their applications
    Venil, C.K.; Zakaria, Z.A.; Ahmad, W.A.
  • Pathway engineering strategies for production of beneficial carotenoids in microbial hosts
    Victor, M.Y.; Bhatia, S.K.
  • Carotenoids and health in older people
    Woodside, J.V.; McGrath, A.J.; Lyner, N.; McKinley, M.C.
  • The final development related microbial pigments and the application in food industry
    Yangilar, F.; Yildiz, P.O.
  • Antioxidant and prooxidant properties of carotenoids
    Young, A.J.; Lowe, G.M.