Flow in a rapidly rotating cone‐shaped PCR‐tube

Flow in a rapidly rotating cone‐shaped PCR‐tube Purpose – A precise and rapid temperature cycling of a small volume of fluid is vital for an effective DNA replication process using the polymerase chain reaction (PCR). The purpose of this paper is to study the velocity and temperature fields inside a rotating PCR‐tube during cooling of the enclosed liquid. Design/methodology/approach – The velocity and temperature fields inside a rotating PCR‐tube during cooling of the enclosed liquid are studied. By using computational fluid dynamics, the time development of the flow can be investigated in detail. Owing to the rotation, the flow exhibits features which could never arise in a non‐rotating system. Findings – An intricate azimuthal boundary layer flow is presented and explained. The inherent problem of stratification of the temperature is discussed, and different methods towards a remedy are presented. By analyzing the governing equations, some properties of the flow observed in the simulations are explained. It is shown that increasing the rate of rotation does not improve temperature homogenization. Research limitations/implications – The simulations were performed for a limited number of temperature boundary conditions, as well as a specific simulation geometry. Practical implications – The analytical and simulation results offer fundamental insight into the physics behind increased DNA duplication. Further simulations offer possible design improvements. Originality/value – While many studies have probed the effects of buoyancy in rotating cylinders and the development of boundary layers in stratified flows in conical containers rotating around their axis of symmetry, little work has been specifically focused on the case where the axis of rotation is normal to the direction of the stratification, which is the case in the present study. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Numerical Methods for Heat & Fluid Flow Emerald Publishing

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Publisher
Emerald Publishing
Copyright
Copyright © 2011 Emerald Group Publishing Limited. All rights reserved.
ISSN
0961-5539
DOI
10.1108/09615531111148473
Publisher site
See Article on Publisher Site

Abstract

Purpose – A precise and rapid temperature cycling of a small volume of fluid is vital for an effective DNA replication process using the polymerase chain reaction (PCR). The purpose of this paper is to study the velocity and temperature fields inside a rotating PCR‐tube during cooling of the enclosed liquid. Design/methodology/approach – The velocity and temperature fields inside a rotating PCR‐tube during cooling of the enclosed liquid are studied. By using computational fluid dynamics, the time development of the flow can be investigated in detail. Owing to the rotation, the flow exhibits features which could never arise in a non‐rotating system. Findings – An intricate azimuthal boundary layer flow is presented and explained. The inherent problem of stratification of the temperature is discussed, and different methods towards a remedy are presented. By analyzing the governing equations, some properties of the flow observed in the simulations are explained. It is shown that increasing the rate of rotation does not improve temperature homogenization. Research limitations/implications – The simulations were performed for a limited number of temperature boundary conditions, as well as a specific simulation geometry. Practical implications – The analytical and simulation results offer fundamental insight into the physics behind increased DNA duplication. Further simulations offer possible design improvements. Originality/value – While many studies have probed the effects of buoyancy in rotating cylinders and the development of boundary layers in stratified flows in conical containers rotating around their axis of symmetry, little work has been specifically focused on the case where the axis of rotation is normal to the direction of the stratification, which is the case in the present study.

Journal

International Journal of Numerical Methods for Heat & Fluid FlowEmerald Publishing

Published: Aug 9, 2011

Keywords: Rotation; Computational fluid mechanics; Coriolis effects; Biomedical flow

References

  • Ranque‐Hilsch vortex tube thermocycler for fast DNA amplification and real‐time optical detection
    Ebmeier, R.J.; Whitney, S.E.; Sarkar, A.; Nelson, M.; Padhye, N.V.; Gogos, G.; Viljohen, H.
  • Buoyant heat transfer in a rotating cup‐like cavity
    Kim, W.N.; Kwak, H.S.; Hyun, J.M.

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