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Effect of legume dietary fiber on rat disaccharidase activity in vitro

Effect of legume dietary fiber on rat disaccharidase activity in vitro Purpose – Brush border intestinal disaccharidases (maltase, sucrase and lactase) play an important role in carbohydrate assimilation. These enzymes are located on the brush border and may interact with legume seed components such as dietary fiber and polyphenols. The purpose of this paper is to assess the effect of legume dietary fiber on rat disaccharidase in vitro . Design/methodology/approach – Rat intestinal disaccharidases from Sprague‐Dawley rats fed a basal diet for 21 days were partially purified from intestinal scrapings. Enzyme activities were tested in vitro in the absence and presence of total dietary fiber isolated from Phaseolus vulgaris (Varieties Tacarigua and Montalbán) and Vigna unguiculata (Varieties Unare and Tuy) seeds that differ in fiber and polyphenol content. Dietary fiber was purified using the AOAC method. Findings – The specific activities of the intestinal brush‐border disaccharidases from rats fed the basal diet were 3.29 ± 0.06, 3.13 ± 0.62 and 0.18 ± 0.04 u moles of glucose released/mg of protein/min for maltase, sucrase and lactase, respectively. Total dietary fiber from the legume tested inhibited both sucrase and maltase. Fiber from the four seeds tested affected sucrase similarly (average inhibition 35 per cent) whereas the fiber residue from P. vulgaris Montalbán was more effective on maltase (26.7 per cent) than that from P. vulgaris Tacarigua (12.2 per cent). Effect of V. unguiculata fibers on maltase was similar and somewhat in between those from P. vulgaris . Originality/value – These results suggest that dietary fiber, as well as other factors from beans with anti‐physiological effect, such as condensed tannins and fitic acid possibly associated with the dietary fiber, may impair carbohydrate availability and may contribute to the low glycemic index proper of these foodstuffs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nutrition & Food Science Emerald Publishing

Effect of legume dietary fiber on rat disaccharidase activity in vitro

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Publisher
Emerald Publishing
Copyright
Copyright © 2008 Emerald Group Publishing Limited. All rights reserved.
ISSN
0034-6659
DOI
10.1108/00346650810891379
Publisher site
See Article on Publisher Site

Abstract

Purpose – Brush border intestinal disaccharidases (maltase, sucrase and lactase) play an important role in carbohydrate assimilation. These enzymes are located on the brush border and may interact with legume seed components such as dietary fiber and polyphenols. The purpose of this paper is to assess the effect of legume dietary fiber on rat disaccharidase in vitro . Design/methodology/approach – Rat intestinal disaccharidases from Sprague‐Dawley rats fed a basal diet for 21 days were partially purified from intestinal scrapings. Enzyme activities were tested in vitro in the absence and presence of total dietary fiber isolated from Phaseolus vulgaris (Varieties Tacarigua and Montalbán) and Vigna unguiculata (Varieties Unare and Tuy) seeds that differ in fiber and polyphenol content. Dietary fiber was purified using the AOAC method. Findings – The specific activities of the intestinal brush‐border disaccharidases from rats fed the basal diet were 3.29 ± 0.06, 3.13 ± 0.62 and 0.18 ± 0.04 u moles of glucose released/mg of protein/min for maltase, sucrase and lactase, respectively. Total dietary fiber from the legume tested inhibited both sucrase and maltase. Fiber from the four seeds tested affected sucrase similarly (average inhibition 35 per cent) whereas the fiber residue from P. vulgaris Montalbán was more effective on maltase (26.7 per cent) than that from P. vulgaris Tacarigua (12.2 per cent). Effect of V. unguiculata fibers on maltase was similar and somewhat in between those from P. vulgaris . Originality/value – These results suggest that dietary fiber, as well as other factors from beans with anti‐physiological effect, such as condensed tannins and fitic acid possibly associated with the dietary fiber, may impair carbohydrate availability and may contribute to the low glycemic index proper of these foodstuffs.

Journal

Nutrition & Food ScienceEmerald Publishing

Published: Jul 18, 2008

Keywords: Diet; Food products

References