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A new method of biomolecular recognition of avidin by light scattering of ZnS:Mn nano‐particles

A new method of biomolecular recognition of avidin by light scattering of ZnS:Mn nano‐particles Purpose – The purpose of this paper is to develop a new method for biomolecular recognition based on light scattering of ZnS:Mn nano‐particle functionalised with biotin. Design/methodology/approach – ZnS:Mn nano‐particles was successfully synthesised from quaternary water‐in‐oil micro‐emulsion system. The addition of biotin and the subsequent specific binding events alter the dielectric environment of the nano‐particle, resulting in a spectral shift of the particle plasmon resonance. Cyclohexane was used as oil, Triten X‐100 as surfactant, n‐hexanol as a co‐surfactant and mercaptoethanol for the best linking of biological part to nano‐particle. Measurement of the content of avidin was achieved by detecting the Department of Biomedical Engineering change in the excited emission. For qualitative and quantitative analyses of this product, scanning electron microscopy, transmission electron microscopy, energy dispersive X‐ray spectroscopy and spectrograph techniques were used. Findings – It was observed that with reducing particle size, emission shifted to the lower wave lengths. In addition, with conjugation between avidin and biotin by mercaptoethanol in biologic media, spectral emission decreased. Practical implications – The method developed could be utilised for synthesis of a variety of ZnS:Mn nano‐particles for a wide range of diagnostic applications. Originality/value – The method for biomolecular recognition based on light scattering of ZnS:Mn nano‐particle functionalised with biotin developed was novel. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pigment & Resin Technology Emerald Publishing

A new method of biomolecular recognition of avidin by light scattering of ZnS:Mn nano‐particles

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Publisher
Emerald Publishing
Copyright
Copyright © 2008 Emerald Group Publishing Limited. All rights reserved.
ISSN
0369-9420
DOI
10.1108/03699420810887852
Publisher site
See Article on Publisher Site

Abstract

Purpose – The purpose of this paper is to develop a new method for biomolecular recognition based on light scattering of ZnS:Mn nano‐particle functionalised with biotin. Design/methodology/approach – ZnS:Mn nano‐particles was successfully synthesised from quaternary water‐in‐oil micro‐emulsion system. The addition of biotin and the subsequent specific binding events alter the dielectric environment of the nano‐particle, resulting in a spectral shift of the particle plasmon resonance. Cyclohexane was used as oil, Triten X‐100 as surfactant, n‐hexanol as a co‐surfactant and mercaptoethanol for the best linking of biological part to nano‐particle. Measurement of the content of avidin was achieved by detecting the Department of Biomedical Engineering change in the excited emission. For qualitative and quantitative analyses of this product, scanning electron microscopy, transmission electron microscopy, energy dispersive X‐ray spectroscopy and spectrograph techniques were used. Findings – It was observed that with reducing particle size, emission shifted to the lower wave lengths. In addition, with conjugation between avidin and biotin by mercaptoethanol in biologic media, spectral emission decreased. Practical implications – The method developed could be utilised for synthesis of a variety of ZnS:Mn nano‐particles for a wide range of diagnostic applications. Originality/value – The method for biomolecular recognition based on light scattering of ZnS:Mn nano‐particle functionalised with biotin developed was novel.

Journal

Pigment & Resin TechnologyEmerald Publishing

Published: Jul 4, 2008

Keywords: Crystals; Zinc; Sulphides; Sensors; Semiconductors; Nanotechnology

References