ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering Classical and contemporary approaches for establishing gene function</h5> With the development of new and affordable methods for whole-genome sequencing, and the design and implementation of large genome annotation projects, scientists are poised to deliver upon the promises of the genomic revolution to transform basic science and personalized medicine. The resulting wealth of information presents researchers with a new primary challenge of converting this enormous amount of data into functionally and clinically relevant knowledge. Central to this problem is the need for efficient and reliable methods that enable investigators to determine how genotype influences phenotype. Targeted gene inactivation via homologous recombination is a powerful method capable of providing conclusive information for evaluating gene function [1] . However, the use of this technique has been hampered by several factors, including the low efficiency at which engineered constructs are correctly inserted into the chromosomal target site, the need for time-consuming and labor-insensitive selection/screening strategies, and the potential for adverse mutagenic effects. Targeted gene knockdown by RNAi (see Glossary ) has provided researchers with a rapid, inexpensive, and high-throughput alternative to homologous recombination [2] . However, knockdown by RNAi is incomplete, varies between experiments and laboratories, has unpredictable off-target effects, and provides http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Trends in Biotechnology Elsevier

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

Loading next page...
 
/lp/elsevier/zfn-talen-and-crispr-cas-based-methods-for-genome-engineering-5Huru3jk0S
Publisher
Elsevier
Copyright
Copyright © 2013 Elsevier Ltd
ISSN
0167-7799
D.O.I.
10.1016/j.tibtech.2013.04.004
Publisher site
See Article on Publisher Site

Abstract

Classical and contemporary approaches for establishing gene function</h5> With the development of new and affordable methods for whole-genome sequencing, and the design and implementation of large genome annotation projects, scientists are poised to deliver upon the promises of the genomic revolution to transform basic science and personalized medicine. The resulting wealth of information presents researchers with a new primary challenge of converting this enormous amount of data into functionally and clinically relevant knowledge. Central to this problem is the need for efficient and reliable methods that enable investigators to determine how genotype influences phenotype. Targeted gene inactivation via homologous recombination is a powerful method capable of providing conclusive information for evaluating gene function [1] . However, the use of this technique has been hampered by several factors, including the low efficiency at which engineered constructs are correctly inserted into the chromosomal target site, the need for time-consuming and labor-insensitive selection/screening strategies, and the potential for adverse mutagenic effects. Targeted gene knockdown by RNAi (see Glossary ) has provided researchers with a rapid, inexpensive, and high-throughput alternative to homologous recombination [2] . However, knockdown by RNAi is incomplete, varies between experiments and laboratories, has unpredictable off-target effects, and provides

Journal

Trends in BiotechnologyElsevier

Published: Jul 1, 2013

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off