Zebrafish deadly seven Functions in Neurogenesis

Zebrafish deadly seven Functions in Neurogenesis In a genetic screen, we isolated a mutation that perturbed motor axon outgrowth, neurogenesis, and somitogenesis. Complementation tests revealed that this mutation is an allele of deadly seven ( des ). By creating genetic mosaics, we demonstrate that the motor axon defect is non-cell autonomous. In addition, we show that the pattern of migration for some neural crest cell populations is aberrant and crest-derived dorsal root ganglion neurons are misplaced. Furthermore, our analysis reveals that des mutant embryos exhibit a neurogenic phenotype. We find an increase in the number of primary motoneurons and in the number of three hindbrain reticulospinal neurons: Mauthner cells, RoL2 cells, and MiD3cm cells. We also find that the number of Rohon–Beard sensory neurons is decreased whereas neural crest-derived dorsal root ganglion neurons are increased in number supporting a previous hypothesis that Rohon–Beard neurons and neural crest form an equivalence group during development. Mutations in genes involved in Notch–Delta signaling result in defects in somitogenesis and neurogenesis. We found that overexpressing an activated form of Notch decreased the number of Mauthner cells in des mutants indicating that des functions via the Notch–Delta signaling pathway to control the production of specific cell types within the central and peripheral nervous systems. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Biology Elsevier

Loading next page...
 
/lp/elsevier/zebrafish-deadly-seven-functions-in-neurogenesis-26PKs6S9eD
Publisher
Elsevier
Copyright
Copyright © 2001 Academic Press
ISSN
0012-1606
eISSN
1095-564X
DOI
10.1006/dbio.2001.0381
Publisher site
See Article on Publisher Site

Abstract

In a genetic screen, we isolated a mutation that perturbed motor axon outgrowth, neurogenesis, and somitogenesis. Complementation tests revealed that this mutation is an allele of deadly seven ( des ). By creating genetic mosaics, we demonstrate that the motor axon defect is non-cell autonomous. In addition, we show that the pattern of migration for some neural crest cell populations is aberrant and crest-derived dorsal root ganglion neurons are misplaced. Furthermore, our analysis reveals that des mutant embryos exhibit a neurogenic phenotype. We find an increase in the number of primary motoneurons and in the number of three hindbrain reticulospinal neurons: Mauthner cells, RoL2 cells, and MiD3cm cells. We also find that the number of Rohon–Beard sensory neurons is decreased whereas neural crest-derived dorsal root ganglion neurons are increased in number supporting a previous hypothesis that Rohon–Beard neurons and neural crest form an equivalence group during development. Mutations in genes involved in Notch–Delta signaling result in defects in somitogenesis and neurogenesis. We found that overexpressing an activated form of Notch decreased the number of Mauthner cells in des mutants indicating that des functions via the Notch–Delta signaling pathway to control the production of specific cell types within the central and peripheral nervous systems.

Journal

Developmental BiologyElsevier

Published: Sep 15, 2001

References

  • Anterior–posterior subdivision of the somite in embryonic zebrafish: Implications for motor axon guidance
    Berhnardt, R.R.; Goerlinger, S.; Roose, M.; Schachner, M.
  • Mapping the origin of the avian enteric nervous system with a retroviral marker
    Epstein, M.L.; Mikawa, T.; Brown, A.M.; McFarlin, D.R.
  • Screen for mutations affecting development of Zebrafish neural crest
    Henion, P.D.; Raible, D.W.; Beattie, C.E.; Stoesser, K.L.; Weston, J.A.; Eisen, J.S.
  • Developmental regulation of islet-1 mRNA expression during neuronal differentiation in embryonic zebrafish
    Inoue, A.; Takahashi, M.; Hatta, K.; Hotta, Y.; H., O.
  • Genetic analysis of melanophore development in zebrafish embryos
    Kelsh, R.N.; Schmid, B.; Eisen, J.S.
  • Stages of embryonic development of the zebrafish
    Kimmel, C.B.; Ballard, W.W.; Kimmel, S.R.; Ullmann, B.; Schilling, T.F.
  • Motor axon pathfinding in the peripheral nervous system
    Krull, C.E.; Koblar, S.A.
  • Specific pan-neural crest expression of zebrafish Crestin throughout embryonic development
    Luo, R.; An, M.; Arduini, B.L.; Henion, P.D.
  • Segregation and early dispersal of neural crest cells in the embryonic zebrafish
    Raible, D.W.; Wood, A.; Hodsdon, W.; Henion, P.D.; Weston, J.A.; Eisen, J.S.
  • Somite development in zebrafish
    Stickney, H.L.; Barresi, M.J.; Devoto, S.H.
  • Zebrafish segmentation and pair-rule patterning
    van Eeden, F.J.M.; Holley, S.A.; Haffter, P.; Nusslein-Volhard, C.
  • Three novel notch genes in zebrafish: Implications for vertebrate notch gene evolution and function
    Westin, J.; Lardelli, M.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off