Xebf3 Is a Regulator of Neuronal Differentiation during Primary Neurogenesis in Xenopus

Xebf3 Is a Regulator of Neuronal Differentiation during Primary Neurogenesis in Xenopus During primary neurogenesis in Xenopus , a cascade of helix–loop–helix (HLH) transcription factors regulates neuronal determination and differentiation. While XNeuroD functions at a late step in this cascade to regulate neuronal differentiation, the factors that carry out terminal differentiation are still unknown. We have isolated a new Xenopus member of the Ebf/Olf-1 family of HLH transcription factors, Xebf3 , and provide evidence that, during primary neurogenesis, it regulates neuronal differentiation downstream of XNeuroD . In developing Xenopus embryos, Xebf3 is turned on in the three stripes of primary neurons at stage 15.5, after XNeuroD. In vitro , XEBF3 binds the EBF/OLF-1 binding site and functions as a transcriptional activator. When overexpressed, Xebf3 is able to induce ectopic neurons at neural plate stages and directly convert ectodermal cells into neurons in animal cap explants. Expression of Xebf3 can be activated by XNeuroD both in whole embryos and in animal caps, indicating that this new HLH factor might be regulated by XNeuroD . Furthermore, in animal caps, XNeuroD can activate Xebf3 in the absence of protein synthesis, suggesting that, in vitro , this regulation is direct. Similar to XNeuroD , but unlike Xebf2/Xcoe2 , Xebf3 expression and function are insensitive to Delta/Notch -mediated lateral inhibition. In summary, we conclude that Xebf3 functions downstream of XNeuroD and is a regulator of neuronal differentiation in Xenopus . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Biology Elsevier

Xebf3 Is a Regulator of Neuronal Differentiation during Primary Neurogenesis in Xenopus

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Publisher
Elsevier
Copyright
Copyright © 2001 Academic Press
ISSN
0012-1606
eISSN
1095-564X
DOI
10.1006/dbio.2001.0230
Publisher site
See Article on Publisher Site

Abstract

During primary neurogenesis in Xenopus , a cascade of helix–loop–helix (HLH) transcription factors regulates neuronal determination and differentiation. While XNeuroD functions at a late step in this cascade to regulate neuronal differentiation, the factors that carry out terminal differentiation are still unknown. We have isolated a new Xenopus member of the Ebf/Olf-1 family of HLH transcription factors, Xebf3 , and provide evidence that, during primary neurogenesis, it regulates neuronal differentiation downstream of XNeuroD . In developing Xenopus embryos, Xebf3 is turned on in the three stripes of primary neurons at stage 15.5, after XNeuroD. In vitro , XEBF3 binds the EBF/OLF-1 binding site and functions as a transcriptional activator. When overexpressed, Xebf3 is able to induce ectopic neurons at neural plate stages and directly convert ectodermal cells into neurons in animal cap explants. Expression of Xebf3 can be activated by XNeuroD both in whole embryos and in animal caps, indicating that this new HLH factor might be regulated by XNeuroD . Furthermore, in animal caps, XNeuroD can activate Xebf3 in the absence of protein synthesis, suggesting that, in vitro , this regulation is direct. Similar to XNeuroD , but unlike Xebf2/Xcoe2 , Xebf3 expression and function are insensitive to Delta/Notch -mediated lateral inhibition. In summary, we conclude that Xebf3 functions downstream of XNeuroD and is a regulator of neuronal differentiation in Xenopus .

Journal

Developmental BiologyElsevier

Published: May 15, 2001

References

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