XCoe2, a transcription factor of the Col/Olf-1/EBF family involved in the specification of primary neurons in Xenopus

XCoe2, a transcription factor of the Col/Olf-1/EBF family involved in the specification of... Background: Primary neurogenesis in Xenopus is a model for studying the control of neural cell fate decisions. The specification of primary neurons appears to be driven by transcription factors containing a basic region and a helix–loop–helix (HLH) motif: expression of Xenopus neurogenin-related-1 (X-ngnr-1) defines the three prospective domains of primary neurogenesis, and expression of XNeuroD coincides with neuronal differentiation. The transition between neuronal competence and stable commitment to a neuronal fate remains poorly characterised, however. Results: Drosophila Collier and rodent early B-cell factor/olfactory-1 define a family of HLH transcription factors containing a previously unknown type of DNA-binding domain. We isolated an orthologous gene from Xenopus, Xcoe2 , which is expressed in precursors of primary neurons. Xcoe2 is transcribed after X-ngnr-1 and before XNeuroD . Overexpression of a dominant-negative mutant of XCoe2 prevented neuronal differentiation. Conversely, overexpressed wild-type Xcoe2 could promote ectopic differentiation of neurons, in both the neural plate and the epidermis. In contrast to studies with X-ngnr-1 or XNeuroD , the supernumerary neurons induced by Xcoe2 appeared in a ‘salt-and-pepper’ pattern, resulting from the activation of X-Delta 1 expression and feedback regulation by lateral inhibition. Conclusions: XCoe2 may play a pivotal role in the transcriptional cascade that specifies primary neurons in Xenopus embryos: by maintaining Delta–Notch signalling, XCoe2 stabilises the higher neural potential of selected progenitor cells that express X-ngnr-1 , ensuring the transition between neural competence and irreversible commitment to a neural fate; and it promotes neuronal differentiation by activating XNeuroD expression, directly or indirectly. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Biology Elsevier

XCoe2, a transcription factor of the Col/Olf-1/EBF family involved in the specification of primary neurons in Xenopus

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Science Ltd
ISSN
0960-9822
DOI
10.1016/S0960-9822(98)70084-3
Publisher site
See Article on Publisher Site

Abstract

Background: Primary neurogenesis in Xenopus is a model for studying the control of neural cell fate decisions. The specification of primary neurons appears to be driven by transcription factors containing a basic region and a helix–loop–helix (HLH) motif: expression of Xenopus neurogenin-related-1 (X-ngnr-1) defines the three prospective domains of primary neurogenesis, and expression of XNeuroD coincides with neuronal differentiation. The transition between neuronal competence and stable commitment to a neuronal fate remains poorly characterised, however. Results: Drosophila Collier and rodent early B-cell factor/olfactory-1 define a family of HLH transcription factors containing a previously unknown type of DNA-binding domain. We isolated an orthologous gene from Xenopus, Xcoe2 , which is expressed in precursors of primary neurons. Xcoe2 is transcribed after X-ngnr-1 and before XNeuroD . Overexpression of a dominant-negative mutant of XCoe2 prevented neuronal differentiation. Conversely, overexpressed wild-type Xcoe2 could promote ectopic differentiation of neurons, in both the neural plate and the epidermis. In contrast to studies with X-ngnr-1 or XNeuroD , the supernumerary neurons induced by Xcoe2 appeared in a ‘salt-and-pepper’ pattern, resulting from the activation of X-Delta 1 expression and feedback regulation by lateral inhibition. Conclusions: XCoe2 may play a pivotal role in the transcriptional cascade that specifies primary neurons in Xenopus embryos: by maintaining Delta–Notch signalling, XCoe2 stabilises the higher neural potential of selected progenitor cells that express X-ngnr-1 , ensuring the transition between neural competence and irreversible commitment to a neural fate; and it promotes neuronal differentiation by activating XNeuroD expression, directly or indirectly.

Journal

Current BiologyElsevier

Published: Feb 12, 1998

References

  • The Notch pathway: democracy and aristocracy in the selection of cell fate
    Koplan, R; Turner, D
  • Neurogenic genes and vertebrate neurogenesis
    Lewis, J
  • collier , a novel regulator of Drosophila head development, is expressed in a single mitotic domain
    Crozatier, M; Valle, D; Dubois, L; Ibnsouda, S; Vincent, A
  • The genetics of Caenorhabditis elegans
    Brenner, S
  • Maintenance of neuroepithelial progenitor cells by Delta-Notch signalling in the embryonic chick retina
    Henrique, D; Hirsinger, E; Adam, J; Le Roux, I; Pourquié, O; Ish-Horowicz, D; Lewis, J
  • neurogenins , a novel family of atonal -related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal cell heterogeneity in the developing CNS and PNS
    Sommer, L; Ma, Q; Anderson, D
  • Basic helix-loop-helix genes in neural development
    Lee, J

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