Why Batch Effects Matter in Omics Data, and How to Avoid Them

Why Batch Effects Matter in Omics Data, and How to Avoid Them Effective integration and analysis of new high-throughput data, especially gene-expression and proteomic-profiling data, are expected to deliver novel clinical insights and therapeutic options. Unfortunately, technical heterogeneity or batch effects (different experiment times, handlers, reagent lots, etc.) have proven challenging. Although batch effect-correction algorithms (BECAs) exist, we know little about effective batch-effect mitigation: even now, new batch effect-associated problems are emerging. These include false effects due to misapplying BECAs and positive bias during model evaluations. Depending on the choice of algorithm and experimental set-up, biological heterogeneity can be mistaken for batch effects and wrongfully removed. Here, we examine these emerging batch effect-associated problems, propose a series of best practices, and discuss some of the challenges that lie ahead. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Trends in Biotechnology Elsevier

Why Batch Effects Matter in Omics Data, and How to Avoid Them

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0167-7799
D.O.I.
10.1016/j.tibtech.2017.02.012
Publisher site
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Abstract

Effective integration and analysis of new high-throughput data, especially gene-expression and proteomic-profiling data, are expected to deliver novel clinical insights and therapeutic options. Unfortunately, technical heterogeneity or batch effects (different experiment times, handlers, reagent lots, etc.) have proven challenging. Although batch effect-correction algorithms (BECAs) exist, we know little about effective batch-effect mitigation: even now, new batch effect-associated problems are emerging. These include false effects due to misapplying BECAs and positive bias during model evaluations. Depending on the choice of algorithm and experimental set-up, biological heterogeneity can be mistaken for batch effects and wrongfully removed. Here, we examine these emerging batch effect-associated problems, propose a series of best practices, and discuss some of the challenges that lie ahead.

Journal

Trends in BiotechnologyElsevier

Published: Jun 1, 2017

References

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