Vitamin A-deficient quail embryos have half a hindbrain and other neural defects

Vitamin A-deficient quail embryos have half a hindbrain and other neural defects Background: Retinoic acid (RA) is a morphogenetically active signalling molecule thought to be involved in the development of several embryonic systems (based on its effect when applied in excess and the fact that it can be detected endogenously in embryos). Here, we adopt a novel approach and use the vitamin A-deficient (A − ) quail embryo to ask what defects these embryos show when they develop in the absence of RA, with particular reference to the nervous system. Results We have examined the anatomy, the expression domains of a variety of genes and the immunoreactivity to several antibodies in these A − embryos. In addition to the previously documented cardiovascular abnormalities, we find that the somites are smaller in A − embryos, otic vesicle development is abnormal and the somites continue up to and underneath the otic vesicle. In the central nervous system, we find that neural crest cells need RA for normal development and survival, and the neural tube fails to extend any neurites into the periphery. Using general hindbrain morphology and the expression patterns of Hoxa -2, Hoxb -1, Hoxb -4, Krox -20 and FGF-3 as markers, we conclude that segmentation in the myelencephalon (rhombomeres 4–8) is disrupted. In contrast, the dorsoventral axis of the neural tube using Shh , islet-1 and Pax -3 as markers is normal. Conclusion These results demonstrate at least three roles for RA in central nervous system development: neural crest survival, neurite outgrowth and hindbrain patterning. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Biology Elsevier

Vitamin A-deficient quail embryos have half a hindbrain and other neural defects

Loading next page...
 
/lp/elsevier/vitamin-a-deficient-quail-embryos-have-half-a-hindbrain-and-other-0mxz4w1uBa
Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Science Ltd
ISSN
0960-9822
D.O.I.
10.1016/S0960-9822(02)00509-2
Publisher site
See Article on Publisher Site

Abstract

Background: Retinoic acid (RA) is a morphogenetically active signalling molecule thought to be involved in the development of several embryonic systems (based on its effect when applied in excess and the fact that it can be detected endogenously in embryos). Here, we adopt a novel approach and use the vitamin A-deficient (A − ) quail embryo to ask what defects these embryos show when they develop in the absence of RA, with particular reference to the nervous system. Results We have examined the anatomy, the expression domains of a variety of genes and the immunoreactivity to several antibodies in these A − embryos. In addition to the previously documented cardiovascular abnormalities, we find that the somites are smaller in A − embryos, otic vesicle development is abnormal and the somites continue up to and underneath the otic vesicle. In the central nervous system, we find that neural crest cells need RA for normal development and survival, and the neural tube fails to extend any neurites into the periphery. Using general hindbrain morphology and the expression patterns of Hoxa -2, Hoxb -1, Hoxb -4, Krox -20 and FGF-3 as markers, we conclude that segmentation in the myelencephalon (rhombomeres 4–8) is disrupted. In contrast, the dorsoventral axis of the neural tube using Shh , islet-1 and Pax -3 as markers is normal. Conclusion These results demonstrate at least three roles for RA in central nervous system development: neural crest survival, neurite outgrowth and hindbrain patterning.

Journal

Current BiologyElsevier

Published: Apr 1, 1996

References

  • Endogenous retinoids in the zebrafish embryo and adult
    Costaridis, P; Horton, C; Zeitlinger, J; Holder, N; Maden, M
  • Endogenous distribution of retinoids during normal development and teratogenesis in the mouse embryo
    Horton, C; Maden, M
  • Comparison of Hensen's node and retinoic acid in secondary axis induction in the early chick embryo
    Chen, Y-P; Solursh, M
  • Hensen's node from vitamin A-deficient quail embryo induces chick limb bud duplication and retains its normal asymmetric expression of Sonic hedgehog ( Shh )
    Chen, Y-P; Dong, D; Kostetskii, I; Zile, MH
  • A series of normal stages in the development of the chick embryo
    Hamburger, V; Hamilton, HL

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off