Visualization of the maturation transition in bacteriophage P22 by electron cryomicroscopy 1 1 Edited by W. Baumeister

Visualization of the maturation transition in bacteriophage P22 by electron cryomicroscopy 1 1... Large-scale conformational transitions are involved in the life-cycle of many types of virus. The dsDNA phages, herpesviruses, and adenoviruses must undergo a maturation transition in the course of DNA packaging to convert a scaffolding-containing precursor capsid to the DNA-containing mature virion. This conformational transition converts the procapsid, which is smaller, rounder, and displays a distinctive skewing of the hexameric capsomeres, to the mature virion, which is larger and more angular, with regular hexons. We have used electron cryomicroscopy and image reconstruction to obtain 15 Å structures of both bacteriophage P22 procapsids and mature phage. The maturation transition from the procapsid to the phage results in several changes in both the conformations of the individual coat protein subunits and the interactions between neighboring subunits. The most extensive conformational transformation among these is the outward movement of the trimer clusters present at all strict and local 3-fold axes on the procapsid inner surface. As the trimer tips are the sites of scaffolding binding, this helps to explain the role of scaffolding protein in regulating assembly and maturation. We also observe DNA within the capsid packed in a manner consistent with the spool model. These structures allow us to suggest how the binding interactions of scaffolding and DNA with the coat shell may act to control the packaging of the DNA into the expanding procapsids. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Biology Elsevier

Visualization of the maturation transition in bacteriophage P22 by electron cryomicroscopy 1 1 Edited by W. Baumeister

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Publisher
Elsevier
Copyright
Copyright © 2000 Academic Press
ISSN
0022-2836
DOI
10.1006/jmbi.2000.3601
Publisher site
See Article on Publisher Site

Abstract

Large-scale conformational transitions are involved in the life-cycle of many types of virus. The dsDNA phages, herpesviruses, and adenoviruses must undergo a maturation transition in the course of DNA packaging to convert a scaffolding-containing precursor capsid to the DNA-containing mature virion. This conformational transition converts the procapsid, which is smaller, rounder, and displays a distinctive skewing of the hexameric capsomeres, to the mature virion, which is larger and more angular, with regular hexons. We have used electron cryomicroscopy and image reconstruction to obtain 15 Å structures of both bacteriophage P22 procapsids and mature phage. The maturation transition from the procapsid to the phage results in several changes in both the conformations of the individual coat protein subunits and the interactions between neighboring subunits. The most extensive conformational transformation among these is the outward movement of the trimer clusters present at all strict and local 3-fold axes on the procapsid inner surface. As the trimer tips are the sites of scaffolding binding, this helps to explain the role of scaffolding protein in regulating assembly and maturation. We also observe DNA within the capsid packed in a manner consistent with the spool model. These structures allow us to suggest how the binding interactions of scaffolding and DNA with the coat shell may act to control the packaging of the DNA into the expanding procapsids.

Journal

Journal of Molecular BiologyElsevier

Published: Mar 31, 2000

References

  • The DNA translocating vertex of dsDNA bacteriophage
    Bazinet, C; King, J
  • DNA packaging in dsDNA bacteriophages
    Black, L.W
  • Proteolytic and conformational control of virus capsid maturation
    Conway, J.F; Duda, R.L; Hendrix, R.W; Steven, A.C
  • Cryo-electron microscopy of vitrified specimens
    Dubochet, J; Adrian, M; Chang, J.J; Homo, J.C; Lepault, J; McDowall, A.W; Schultz, P
  • Capsid expansion follows the initiation of DNA packaging in bacteriophage T4
    Jardine, P.J; Coombs, D.H
  • The bacteriophage T4 DNA packaging apparatus targets the unexpanded prohead
    Jardine, P.J; McCormick, M.C; Lutze-Wallace, C; Coombs, D.H
  • Three-dimensional structure of scaffolding-containing phage P22 procapsids by electron cryo-microscopy
    Thuman-Commike, P.A; Greene, B; Jakana, J; Prasad, B.V; King, J; Prevelige, P.E; Chiu, W
  • CTF determination of images of ice-embedded single particles using a graphics interface
    Zhou, Z.H; Hardt, S; Wang, B; Sherman, M.B; Jakana, J; Chiu, W

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