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, Wuertz S, Thompson JR Variably improved microbial source tracking with digital droplet PCR Water Res 2019 159 192 202 10.1016/j.watres.2019.04.056 Google Scholar Crossref Search ADS PubMed WorldCat 27 ...
of PCR primers for the genomic regions of interest are segregated in water in emulsion droplets and then pooled to create a “primer library.” Separately, emulsion droplets containing genomic DNA and PCR ...
approach has enabled researchers to determine population level microheterogeneities (Blainey et al., 2011), study cell-to-cell interactions (Yoon et al., 2011), improve phylogenetic resolution of microbial ...
quantification techniques. We evaluate pulsed-field gel electrophoresis, droplet digital PCR , and Nextera-based whole genome sequencing as approaches to copy number estimation, comparing techniques across model ...
Moderate Quantitative PCR / droplet digital PCR Low High Low High Low Loop-mediated isothermal amplification Low High Low Low Low Metabarcoding Low High High Very high Low Identification method Taxonomic ...
source of artefactual recombination when generating PCR -amplified cDNA for sequencing of HIV (39,40) and other RNA viruses. To counteract this major PCR artefact we adapted established emulsion PCR (emPCR ...
issues by employing novel amplification technologies adapted to degraded DNA or by using more sensitive DNA detection technologies such as NGS, digital PCR (dPCR) and pyrosequencing. Redundant diagnostics ...
wasps in a plastic petri dish in a droplet of PBS. Light images of representative ovaries were generated on a Leica MZLFIII dissecting stereomicroscope with a Leica DFC420 digital camera. For Transmission ...
-Candida innate host defense. Population-genetic approaches have translated several findings to humans with the prospect of devising improved individualized risk stratification and prognostication strategies ...
as the sole carbon source , compared with the wild-type strain. The cut2 mutant strain is severely less pathogenic than the wild type or complemented cut2/CUT2 strain on rice (Oryza sativa) and barley (Hordeum ...
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