•HSE and mtDNA analysis for short amplicon lengths was complete to 219 pg of total DNA input.•HSE and the subsequent midi-mito assay proved to be stable against DNA degradation.•HSE allows 10-fold-plus increase of a 5% minor component in a mixture.•HSE of mtDNA mixtures was successfully validated for forensic casework.
Forensic Science International: Genetics – Elsevier
Published: Jul 1, 2018
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