Utility of Acridine Orange staining for detection of bacteria from positive blood cultures

Utility of Acridine Orange staining for detection of bacteria from positive blood cultures The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal Of Microbiological Methods Elsevier

Utility of Acridine Orange staining for detection of bacteria from positive blood cultures

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0167-7012
eISSN
1872-8359
D.O.I.
10.1016/j.mimet.2017.06.014
Publisher site
See Article on Publisher Site

Abstract

The diagnostic performance of AO stain was evaluated for the detection of bacteria and or fungi from positive blood cultures. The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. The specificity of both the stains was 100%. Overall agreement between the two stains was 98.23% (688/700). The organisms that were missed by GS and positive by AO were Candida species (Sutton, 2006) and Gram negative bacilli (GNB) (Sutton, 2006). Sensitivity of GS was 82.35% and AO was 100% among mixed cultures. Immediate reporting of the results of AO stain would have a significant impact on clinical management of patients with serious blood stream infections.

Journal

Journal Of Microbiological MethodsElsevier

Published: Aug 1, 2017

References

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