Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese

Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft... The aim of this work was to develop an assay capable of detecting adulteration of soft goat, sheep and buffalo milk cheese with bovine milk from cheaper sources. A previously developed indirect competitive ELISA had a lower sensitivity when applied to cheese, compared with milk. A sandwich ELISA was developed utilising the same monoclonal antibody in combination with a polyclonal goat anti-bovine IgG antibody. Once optimised, the ELISA was found to be highly specific. Detection limits in milk were 0.001% cows’ milk adulteration of sheep or buffalo milk, and 0.01% cows’ milk adulteration of goat milk. Detection limits in soft cheese were 0.001% in goat cheese and 0.01% in sheep or buffalo cheese. The assay was highly reproducible with both intra- and inter-assay coefficient of variation <10%. The ELISA performance makes it suitable for development as a kit for use in routine surveillance of milk and soft cheese. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Dairy Journal Elsevier

Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese

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Publisher
Elsevier
Copyright
Copyright © 2005 Elsevier Ltd
ISSN
0958-6946
eISSN
1879-0143
D.O.I.
10.1016/j.idairyj.2005.07.009
Publisher site
See Article on Publisher Site

Abstract

The aim of this work was to develop an assay capable of detecting adulteration of soft goat, sheep and buffalo milk cheese with bovine milk from cheaper sources. A previously developed indirect competitive ELISA had a lower sensitivity when applied to cheese, compared with milk. A sandwich ELISA was developed utilising the same monoclonal antibody in combination with a polyclonal goat anti-bovine IgG antibody. Once optimised, the ELISA was found to be highly specific. Detection limits in milk were 0.001% cows’ milk adulteration of sheep or buffalo milk, and 0.01% cows’ milk adulteration of goat milk. Detection limits in soft cheese were 0.001% in goat cheese and 0.01% in sheep or buffalo cheese. The assay was highly reproducible with both intra- and inter-assay coefficient of variation <10%. The ELISA performance makes it suitable for development as a kit for use in routine surveillance of milk and soft cheese.

Journal

International Dairy JournalElsevier

Published: Jul 1, 2006

References

  • Detection and quantification of goat's cheese in ewe's cheese using a monoclonal antibody and two ELISA formats
    Haza, A.I.; Morales, P.; Martin, R.; Garcia, T.; Anguita, G.; Sanz, B.; Hernandez, P.E.
  • Application of immunological methods for the detection of species adulteration in dairy products
    Hurley, I.P.; Ireland, H.E.; Coleman, R.C.; Williams, J.H.H.
  • Recent developments in antibody-based analytical methods for the differentiation of milk from different species
    Moatsou, G.; Anifantakis, E.
  • Detection of caprine casein in ovine Halloumi cheese
    Moatsou, G.; Hatzinaki, A.; Psathas, G.; Anifantakis, E.
  • Analysis of cows', ewes' and goats' milk mixtures by capillary electrophoresis: Quantification by multivariate regression analysis
    Molina, E.; Martin-Alvarez, P.J.; Ramos, M.
  • Influences on diet, health behaviours and their outcome in select ethnocultural and religious groups
    Shatenstein, B.; Ghadirian, P.

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