Biosensors and Bioelectronics 116 (2018) 108–115 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: www.elsevier.com/locate/bios Ultrasensitive dual probe immunosensor for the monitoring of nicotine induced-brain derived neurotrophic factor released from cancer cells a a a a,b a, Mahmood H. Akhtar , Khalil K. Hussain , N.G. Gurudatt , Pranjal Chandra , Yoon-Bo Shim Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India ARTIC L E I NF O ABSTRAC T Keywords: Brain-derived neurotrophic factor (BDNF) was detected in the extracellular matrix of neuronal cells using a dual Immunosensor probe immunosensor (DPI), where one of them was used as a working and another bioconjugate loading probe. Neurotrophins The working probe was fabricated by covalently immobilizing capture anti-BDNF (Cap Ab) on the gold nano- Brain-derived neurotrophic factor particles (AuNPs)/conducting polymer composite layer. The bioconjugate probe was modiﬁed by drop casting a Dual probe sensor bioconjugate particles composed of conducting polymer self-assembled AuNPs, immobilized with detection anti- Bioconjugate particles BDNF (Det Ab) and toluidine blue O (TBO). Each sensor layer was characterized using the surface analysis and electrochemical
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