Typing of European strains of parvovirus B19 by restriction endonuclease analyses and sequencing: Identification of evolutionary lineages and evidence of recombination of markers from different lineages

Typing of European strains of parvovirus B19 by restriction endonuclease analyses and sequencing:... European isolates of parvovirus B19 were analyzed by restriction enzyme analysis of PCR products of the VP1/2 coding region and sequencing of the same amplified region, five cloned fragments from each PCR product. Two main groupings were found based on three perfectly linked point deviations. On the assumption that identical point deviations causing the various restriction patterns regardless of time and origin of virus isolation were unlikely to emerge independently in different evolutionary lineages, traits of evolutionary lineages were identified, suggesting a clonal population structure of global circulating B19 strains. However, combinations of markers from different evolutionary lineages were also found, particularly in a strain derived from an individual chronically infected with B19 for more than 7 years. As chronically infected individuals might be subject to superinfections due to contacts or possibly due to blood transfusions or the administration of γ -globulin, it is suggested that coexistance of, and recombination between variants of B19 of different phylogenetic origin incidentally occur in such individuals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Typing of European strains of parvovirus B19 by restriction endonuclease analyses and sequencing: Identification of evolutionary lineages and evidence of recombination of markers from different lineages

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Publisher
Elsevier
Copyright
Copyright © 1998 Elsevier Science B.V.
ISSN
0168-1702
DOI
10.1016/S0168-1702(97)00151-2
Publisher site
See Article on Publisher Site

Abstract

European isolates of parvovirus B19 were analyzed by restriction enzyme analysis of PCR products of the VP1/2 coding region and sequencing of the same amplified region, five cloned fragments from each PCR product. Two main groupings were found based on three perfectly linked point deviations. On the assumption that identical point deviations causing the various restriction patterns regardless of time and origin of virus isolation were unlikely to emerge independently in different evolutionary lineages, traits of evolutionary lineages were identified, suggesting a clonal population structure of global circulating B19 strains. However, combinations of markers from different evolutionary lineages were also found, particularly in a strain derived from an individual chronically infected with B19 for more than 7 years. As chronically infected individuals might be subject to superinfections due to contacts or possibly due to blood transfusions or the administration of γ -globulin, it is suggested that coexistance of, and recombination between variants of B19 of different phylogenetic origin incidentally occur in such individuals.

Journal

Virus ResearchElsevier

Published: Feb 1, 1998

References

  • Parvovirus B19 for the hematologist
    Harris, J.W.
  • Identification of human parvovirus B19 infection in idiopathic nonimmune hydrops fetalis
    Jordan, J.A.
  • Partial nucleotide sequencing and characterization of human parvovirus B19 genome DNA from damaged human fetuses and from patients with leukemia
    Umene, K.; Nunoue, T.

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