Molecular Phylogenetics and Evolution 101 (2016) 176–193 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Two DNA barcodes and morphology for multi-method species delimitation in Bonnetina tarantulas (Araneae: Theraphosidae) David Ortiz , Oscar F. Francke Colección Nacional de Arácnidos, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, Mexico ar ti c l e i nf o ab stra ct Article history: Determining species boundaries is a central debate in biology. Several recently developed molecular Received 1 April 2015 delimitation methods have highlighted extensive inconsistency in classical morphological taxonomy. Revised 7 April 2016 However, choosing between them is contentious. Molecular studies on theraphosid spiders have found Accepted 1 May 2016 considerable cryptic diversity and many species redundantly described. Most of these studies have relied Available online 03 May 2016 only on COI, a mitochondrial marker that has proven its efﬁcacy in animal studies, but which also might lead to an over-estimation of diversity. Keywords: Here we present an integrative approach to species delimitation in Bonnetina, a poorly known group of Species delimitation tarantulas endemic to Mexico. We employed morphological evidence, as well as different setups with DNA barcoding distance-based (Hard-Gap barcoding and ABGD)
Molecular Phylogenetics and Evolution – Elsevier
Published: Aug 1, 2016
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