Journal of Colloid and Interface Science 461 (2016) 419–424 Contents lists available at ScienceDirect Journal of Colloid and Interface Science journal homepage: www.elsevier.com/locate/jcis Trypsin and trypsin inhibitor bind PAMAM nanoparticles: Effect of hydrophobicity on protein–polymer conjugation P. Chanphai, H.A. Tajmir-Riahi Department of Chemistry-Biochemistry and Physics, University of Québec at Trois Rivières, C.P. 500, TR, Quebec G9A 5H7, Canada highl i ghts graphical a bstrac t Conjugations of trypsin and trypsin inhibitor with PAMAM-G4 nanoparticles are studied here. Protein–polymer interactions are via H-bonding and van der Waals contacts. Trypsin inhibitor forms stronger polymer adduct than trypsin. Hydrophobicity plays a role in polymer–protein conjugation. Polymer interaction alters protein conformation. article i nfo abstract Article history: Protein–polymer conjugates are widely used in therapeutic drug delivery. We report the bindings of Received 18 August 2015 trypsin (Try) and trypsin inhibitor (Tryi) with polyamidoamine (PAMAM-G4) dendrimer at physiological Revised 20 September 2015 conditions, using thermodynamic analysis, UV–Visible and Fourier transform infrared (FTIR) spectro- Accepted 21 September 2015 scopic methods. Thermodynamic parameters DS, DH and DG showed protein–PAMAM bindings occur Available online 25 September 2015 via H-bonding and van der Waals contacts with trypsin inhibitor forming more stable conjugate than trypsin. PAMAM complexation induces more
Journal of Colloid and Interface Science – Elsevier
Published: Jan 1, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera