Trueness and precision of the real-time RT-PCR method for quantifying the chronic bee paralysis virus genome in bee homogenates evaluated by a comparative inter-laboratory study

Trueness and precision of the real-time RT-PCR method for quantifying the chronic bee paralysis... •Chronic bee paralysis is diagnosed by quantifying the causative virus, CBPV, in honeybees by real-time PCR•21 laboratories assayed honeybee homogenates, adjusted to three virus loads (5, 8, and 9 log10 CBPV genome copies per bee)•The accuracy (trueness and precision) of the method implemented in the laboratories was evaluated according to international standards•The inter-laboratory confidence of viral quantification was about+/− 2 log10 for a diagnostic threshold of 8 log10 CBPV genome copies per bee•Taking into account this confidence, viral loads over 6 log10 CBPV RNA copies per bee may indicate probable cases of chronic paralysis http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2017 The Authors
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.07.013
Publisher site
See Article on Publisher Site

Abstract

•Chronic bee paralysis is diagnosed by quantifying the causative virus, CBPV, in honeybees by real-time PCR•21 laboratories assayed honeybee homogenates, adjusted to three virus loads (5, 8, and 9 log10 CBPV genome copies per bee)•The accuracy (trueness and precision) of the method implemented in the laboratories was evaluated according to international standards•The inter-laboratory confidence of viral quantification was about+/− 2 log10 for a diagnostic threshold of 8 log10 CBPV genome copies per bee•Taking into account this confidence, viral loads over 6 log10 CBPV RNA copies per bee may indicate probable cases of chronic paralysis

Journal

Journal of Virological MethodsElsevier

Published: Oct 1, 2017

References

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