Trace analysis of 61 natural and synthetic progestins in river water and sewage effluents by ultra-high performance liquid chromatography–tandem mass spectrometry

Trace analysis of 61 natural and synthetic progestins in river water and sewage effluents by... A broad number of natural and synthetic progestins are widely used in human and veterinary therapies. Although progestins exhibit adverse effects in aquatic organisms, information about environmental occurrence and fate have been limited to several compounds, hampering the accuracy of risk assessments of the compounds. In this study, a selective and sensitive analytical method was established to simultaneously determine 19 natural and 42 synthetic progestins in environmental waters, and the synthetic progestins included 19-nortestosterone, 17α-hydroxyprogesterone and progesterone derivatives. All of the target compounds were effectively separated using an HSS T3 column, and the recoveries for effluent and river samples were 80–115% and 75–105%, respectively. The detection limits for the 61 analytes were in the range of 0.05–0.60 ng/L and 0.03–0.40 ng/L for the effluent and river samples, respectively. The developed method is applied to analyze the target progestogens in sewage effluent and river water samples from Beijing. The detected concentrations of natural progesterone metabolites (3α-hydroxy-5β-tetrahydroprogesterone) were up to 63 times higher than those of the parent compound. Of the three groups of synthetic progestins, the progesterone derivatives were detected for the first time and had the highest concentrations followed by the 19-nortestosterone and 17α-hydroxyprogesterone derivatives. In contrast to previous studies, the predominant derivative compounds of 19-nortestosterone were found to be 19-nortestosterone, gestodene and mifepristone, and those of 17α-hydroxyprogesterone were 6-epi-medroxy progesterone 17-acetate and melengestrol acetate. The toxicities and environmental risk of these emerging progestins deserves more attention in the future. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Water Research Elsevier

Trace analysis of 61 natural and synthetic progestins in river water and sewage effluents by ultra-high performance liquid chromatography–tandem mass spectrometry

11 pages
Read Article

Loading next page...
 
/lp/elsevier/trace-analysis-of-61-natural-and-synthetic-progestins-in-river-water-gjQM86Z0dK
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0043-1354
DOI
10.1016/j.watres.2018.01.030
Publisher site
See Article on Publisher Site

Abstract

A broad number of natural and synthetic progestins are widely used in human and veterinary therapies. Although progestins exhibit adverse effects in aquatic organisms, information about environmental occurrence and fate have been limited to several compounds, hampering the accuracy of risk assessments of the compounds. In this study, a selective and sensitive analytical method was established to simultaneously determine 19 natural and 42 synthetic progestins in environmental waters, and the synthetic progestins included 19-nortestosterone, 17α-hydroxyprogesterone and progesterone derivatives. All of the target compounds were effectively separated using an HSS T3 column, and the recoveries for effluent and river samples were 80–115% and 75–105%, respectively. The detection limits for the 61 analytes were in the range of 0.05–0.60 ng/L and 0.03–0.40 ng/L for the effluent and river samples, respectively. The developed method is applied to analyze the target progestogens in sewage effluent and river water samples from Beijing. The detected concentrations of natural progesterone metabolites (3α-hydroxy-5β-tetrahydroprogesterone) were up to 63 times higher than those of the parent compound. Of the three groups of synthetic progestins, the progesterone derivatives were detected for the first time and had the highest concentrations followed by the 19-nortestosterone and 17α-hydroxyprogesterone derivatives. In contrast to previous studies, the predominant derivative compounds of 19-nortestosterone were found to be 19-nortestosterone, gestodene and mifepristone, and those of 17α-hydroxyprogesterone were 6-epi-medroxy progesterone 17-acetate and melengestrol acetate. The toxicities and environmental risk of these emerging progestins deserves more attention in the future.

Journal

Water Research – Elsevier

Published: Apr 15, 2018

References

  • Multi-residue analytical method for human pharmaceuticals and synthetic hormones in river water and sewage effluents by solid-phase extraction and liquid chromatography-tandem mass spectrometry
    Al-Odaini, N.A.; Zakaria, M.P.; Yaziz, M.I.; Surif, S.
  • Environmental factors affecting ultraviolet photodegradation rates and estrogenicity of estrone and ethinylestradiol in natural waters
    Atkinson, S.K.; Marlatt, V.L.; Kimpe, L.E.; Lean, D.R.S.; Trudeau, V.L.; Blais, J.M.
  • Progestogens in menopausal hormone therapy
    Bińkowska, M.; Woroń, J.
  • Trace analysis of androgens and progestogens in environmental waters by ultra-performance liquid chromatography-electrospray tandem mass spectrometry
    Chang, H.; Wu, S.; Hu, J.; Asami, M.; Kunikane, S.
  • Determination and source apportionment of five classes of steroid hormones in urban rivers
    Chang, H.; Wan, Y.; Hu, J.
  • Occurrence of androgens and progestogens in wastewater treatment plants and receiving river waters: comparison to estrogens
    Chang, H.; Wan, Y.; Wu, S.; Fan, Z.; Hu, J.
  • Exposure to the pheromone 17α, 20β-dihydroxy-4-pregnen-3- one enhances the behavioural spawning success, sperm production and sperm motility of male goldfish
    Defraipont, M.; Sorensen, P.W.
  • Determination of sex hormones and nonylphenol ethoxylates in the aqueous matrixes of two pilot-scale municipal wastewater treatment plants
    Esperanza, M.; Suidan, M.T.; Nishimura, F.; Wang, Z.M.; Sorial, G.A.
  • On-line solid-phase extraction coupled to liquid chromatography tandem mass spectrometry optimized for the analysis of steroid hormones in urban wastewaters
    Fayad, P.B.; Prévost, M.; Sauvé, S.
  • Progestins as endocrine disrupters in aquatic ecosystems: concentrations, effects and risk assessment
    Fent, K.
  • An on-line solid phase extraction method coupled with UHPLC-MS/MS for the determination of steroid hormone compounds in treated water samples from waste water treatment plants
    Guedes-Alonso, R.; Sosa-Ferrera, Z.; Santana-Rodríguez, J.J.
  • Influence of acidification on the partitioning of steroid hormones among filtrate, filter media, and retained particulate matter
    Havens, S.M.; Hedman, C.J.; Hemming, J.D.C.; Mieritz, M.G.; Shafer, M.M.; Schauer, J.J.
  • The synthetic progestogen, levonorgestrel, but not natural progesterone, affects male mate calling behavior of Xenopus laevis
    Hoffmann, F.; Kloas, W.
  • In vitro bioassay determination of dioxin-like and estrogenic activity in sediment and water from Ulsan Bay and its vicinity, Korea
    Khim, J.S.; Lee, K.-T.; Villeneuve, D.L.; Kannan, K.; Giesy, J.P.; Koh, C.-H.
  • The challenge presented by progestins in ecotoxicological research: a critical review
    Kumar, V.; Johnson, A.C.; Trubiroha, A.; Jitka Tumová, J.; Ihara, M.; Grabic, R.; Kloas, W.; Tanaka, H.; Kroupová, H.K.
  • Analysis of phytoestrogens, progestogens and estrogens in environmental waters from Rio de Janeiro (Brazil)
    Kuster, M.; Azevedo, D.A.; Lopez de Alda, M.J.; Aquino Neto, F.R.; Barcelo, D.
  • Occurrence of acidic pharmaceuticals in raw and treated sewages and in receiving waters
    Lindqvista, N.; Tuhkanenb, T.; Kronberg, L.
  • Trace analysis of 28 steroids in surface water, wastewater and sludge samples by rapid resolution liquid chromatography- electrospray ionization tandem mass spectrometry
    Liu, S.; Ying, G.G.; Zhao, J.L.; Chen, F.; Yang, B.; Zhou, L.J.; Lai, H.
  • Steroids in a typical swine farm and their release into the environment
    Liu, S.; Ying, G.G.; Zhou, L.J.; Zhang, R.Q.; Chen, Z.F.; Lai, H.J.
  • Occurrence and fate of macrolide antibiotics in wastewater treatment plants and in the Glatt Valley watershed, Switzerland
    McArdell, C.S.; Molnar, E.; Suter, M.J.F.; Giger, W.
  • Testing estrogenicity of known and novel (xeno-) estrogens in the MolDarT using developing zebrafish (Danio rerio)
    Muncke, J.; Junghans, M.; Eggen, R.I.
  • Evaluation of BEH C18, BEH HILIC, and HSS T3 (C18) column chemistries for the UPLC-MS-MS analysis of glutathione, glutathione disulfide, and ophthalmic acid in mouse liver and human plasma
    New, L.S.; Chan, E.C.Y.
  • Reproductive responses in fathead minnow and Japanese medaka following exposure to a synthetic progestin, norethindrone
    Paulos, P.; Runnalls, T.J.; Nallani, G.; La Point, T.; Scott, A.P.; Sumpter, J.P.; Huggett, D.B.
  • Liquid chromatography-tandem mass spectrometry determination of nonionic organophosphorus flame retardants and plasticizers in wastewater samples
    Rodil, R.; Quintana, J.B.; Reemtsma, T.
  • Several synthetic progestins with different potencies adversely affect reproduction of fish
    Runnalls, T.J.; Beresford, N.; Losty, E.; Scott, A.P.; Sumpter, J.P.
  • Female specific reproductive toxicity of environmental progestin in amphibians
    Safholm, M.; Fick, J.; Berg, C.
  • Estrogen- induced alterations in amh and dmrt1 expression signal for disruption in male sexual development in the zebrafish
    Schulz, R.W.; Bogerd, J.; Male, R.; Ball, J.; Fenske, M.; Olsen, L.C.; Tyler, C.R.
  • The use of newer progestins for contraception
    Sitruk-Warea, R.; Nath, A.
  • Sulfated 17,20β-dihydroxy-4-pregnen-3-one functions as a potent and specific olfactory stimulant with pheromonal actions in the goldfish
    Sorensen, P.W.; Scott, A.P.; Stacey, N.E.; Bowdin, L.
  • Sexually mature male goldfish release large quantities of androstenedione into the water where it functions as a pheromone
    Sorensen, P.W.; Pinillos, M.; Scott, A.P.
  • Multi-residue analysis of steroids at sub-ng/L levels in surface and ground-waters using liquid chromatography coupled to tandem mass spectrometry
    Vulliet, E.; Wiest, L.; Baudot, R.; Grenier-Loustalot, M.F.
  • Effects of synthetic gestagens on fish reproduction
    Zeilinger, J.; Steger-Hartmann, T.; Maser, E.; Goller, S.; Vonk, R.; Länge, R.
  • Synthetic progestins medroxyprogesterone acetate and dydrogesterone and their binary mixtures adversely affect reproduction and lead to histological and transcriptional alterations in zebrafish (Danio rerio)
    Zhao, Y.; Castiglioni, S.; Fent, K.
  • The steroid pheromone 4-pregnen-17, 20ss-diol-3-one increases fertility and paternity in gold-fish
    Zheng, W.; Strobeck, C.; Stacey, N.
  • Transcriptional and physiological responses induced by binary mixtures of drospirenone and progesterone in zebrafish (Danio rerio) at environmental concentrations
    Zucchi, S.; Mirbahai, L.; Castiglioni, S.; Fent, K.
  • Determination of polar drug residues in sewage and surface water applying liquid chromatography-tandem mass spectrometry
    Zuehlke, S.; Duennbier, U.; Heberer, T.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial