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in either case. One possible explanation might be that flavonoid gene and antisense transcripts are at very low levels in regenerated plant tissues compared with corresponding root cultures . Alternatively ...
Initial screening of Lotus corniculatus for condensed tannin phenotype Standard transformation procedures were employed to introduce a CaMV35S‐Sn gene construct into Lotus corniculatus. Clonal genotypes ...
controversial. Colliver et al. (1997) obtained an increase of CHS expression and CT levels in L. corniculatus hairy root lines transformed with a heterologous CHS gene in antisense orientation. They proposed ...
, Lotus japonicus. A total of five DFR genes were found to form a cluster within a 38 kb region in the L. japonicus genome, whereas six cDNAs, including two splicing variants resulting from a transversion ...
gene of the flavonoid pathway is sufficient to down-regulate the PA pathway in leaves of a high PA genotype of Lotus corniculatus. For this purpose, the FaMYB1 gene from strawberry was used, which has ...
)-dependent reductases and dehydrogenases (Lacombe et al., 1997), is also present in the strawberry putative DFR protein (Fig. 1). Tissue and Developmental Gene Expression The spatial and temporal expression ...
, increases the levels of PAs in the vegetative tissues of L. corniculatus and regulates the expression of DFR and ANS (Paolocci et al., 2005). Here, we report on the cloning and characterization of genes ...
, but the success of this strategy has varied. For example, Sn was shown to stimulate or inhibit proanthocyanidin accumulation in Lotus corniculatus, depending on the tissue type, transformed line, and gene copy ...
. Tissue -Specific Expression of PA Biosynthetic Genes in M. truncatula To assess the potential involvement of MtLAR and MtANS in PA biosynthesis, their transcript levels were first estimated by microarray ...
. tabacum, M. sativa, and T. repens resulted in ectopic accumulation of PAs in leaf tissues of these plants as well as up-regulation of PA biosynthetic pathway genes in T. repens transformants . Silenced ...
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