Time-course expression profile and diagnostic potential of a miRNA panel in exosomes and total serum in acute liver injury

Time-course expression profile and diagnostic potential of a miRNA panel in exosomes and total... International Journal of Biochemistry and Cell Biology 100 (2018) 11–21 Contents lists available at ScienceDirect International Journal of Biochemistry and Cell Biology journal homepage: www.elsevier.com/locate/biocel Time-course expression profile and diagnostic potential of a miRNA panel in exosomes and total serum in acute liver injury a b a b Tarek K. Motawi , Mohamed R. Mohamed , Nancy N. Shahin , Mohamed A.M. Ali , a, May A. Azzam Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt Biochemistry Department, Faculty of Science, Ain Shams University, Egypt ARTIC L E I NF O ABSTRAC T Keywords: Circulating miRNAs have recently emerged as attractive candidates for biomarker discovery. However, they Drug-induced liver injury have a variant distribution in circulation, and the diagnostic significance of their compartmentalization is yet to Acetaminophen be elucidated. This study explored the time-course expression profile and the diagnostic potential of miRNAs- Thioacetamide 122a-5p, 192-5p, 193a-3p and 194-5p in exosomal and total serum compartments in two rat models of acute Extracellular vesicles liver injury (ALI) . Exosomes were isolated and characterized in terms of morphology, size and CD-63 surface Biomarkers marker expression. Exosomal, serum and hepatic miRNAs were quantified using q-RT-PCR. An inverse expres- sion pattern of hepatic and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The International Journal of Biochemistry & Cell Biology Elsevier

Time-course expression profile and diagnostic potential of a miRNA panel in exosomes and total serum in acute liver injury

Loading next page...
 
/lp/elsevier/time-course-expression-profile-and-diagnostic-potential-of-a-mirna-IQeG5anjWT
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
1357-2725
eISSN
1878-5875
D.O.I.
10.1016/j.biocel.2018.05.002
Publisher site
See Article on Publisher Site

Abstract

International Journal of Biochemistry and Cell Biology 100 (2018) 11–21 Contents lists available at ScienceDirect International Journal of Biochemistry and Cell Biology journal homepage: www.elsevier.com/locate/biocel Time-course expression profile and diagnostic potential of a miRNA panel in exosomes and total serum in acute liver injury a b a b Tarek K. Motawi , Mohamed R. Mohamed , Nancy N. Shahin , Mohamed A.M. Ali , a, May A. Azzam Biochemistry Department, Faculty of Pharmacy, Cairo University, Egypt Biochemistry Department, Faculty of Science, Ain Shams University, Egypt ARTIC L E I NF O ABSTRAC T Keywords: Circulating miRNAs have recently emerged as attractive candidates for biomarker discovery. However, they Drug-induced liver injury have a variant distribution in circulation, and the diagnostic significance of their compartmentalization is yet to Acetaminophen be elucidated. This study explored the time-course expression profile and the diagnostic potential of miRNAs- Thioacetamide 122a-5p, 192-5p, 193a-3p and 194-5p in exosomal and total serum compartments in two rat models of acute Extracellular vesicles liver injury (ALI) . Exosomes were isolated and characterized in terms of morphology, size and CD-63 surface Biomarkers marker expression. Exosomal, serum and hepatic miRNAs were quantified using q-RT-PCR. An inverse expres- sion pattern of hepatic and

Journal

The International Journal of Biochemistry & Cell BiologyElsevier

Published: Jul 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off