Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers

Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers Article history: Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to Received 11 November 2011 conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are Received in revised form released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present 14 December 2011 work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact Accepted 21 December 2011 of platelet lysate (PL) with cornea ulcers. Available online 10 January 2012 A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the Keywords: obtained formulation was able to quickly thermogelify at about 32 C and was characterized by good Growth factors mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the Platelet lysate vehicle and stable in the formulation up to 15 days of storage at 2–8 C. Cornea Wound healing In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) Thermogelling showed that the formulation enhanced cell growth and put in evidence a synergistic effect of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Pharmaceutics Elsevier

Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers

Loading next page...
 
/lp/elsevier/thermosensitive-eyedrops-containing-platelet-lysate-for-the-treatment-PgHgrefzgD
Publisher
Elsevier
Copyright
Copyright © 2012 Elsevier B.V.
ISSN
0378-5173
D.O.I.
10.1016/j.ijpharm.2011.12.059
Publisher site
See Article on Publisher Site

Abstract

Article history: Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to Received 11 November 2011 conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are Received in revised form released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present 14 December 2011 work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact Accepted 21 December 2011 of platelet lysate (PL) with cornea ulcers. Available online 10 January 2012 A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the Keywords: obtained formulation was able to quickly thermogelify at about 32 C and was characterized by good Growth factors mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the Platelet lysate vehicle and stable in the formulation up to 15 days of storage at 2–8 C. Cornea Wound healing In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) Thermogelling showed that the formulation enhanced cell growth and put in evidence a synergistic effect of

Journal

International Journal of PharmaceuticsElsevier

Published: Apr 15, 2012

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off