Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers

Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers Article history: Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to Received 11 November 2011 conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are Received in revised form released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present 14 December 2011 work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact Accepted 21 December 2011 of platelet lysate (PL) with cornea ulcers. Available online 10 January 2012 A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the Keywords: obtained formulation was able to quickly thermogelify at about 32 C and was characterized by good Growth factors mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the Platelet lysate vehicle and stable in the formulation up to 15 days of storage at 2–8 C. Cornea Wound healing In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) Thermogelling showed that the formulation enhanced cell growth and put in evidence a synergistic effect of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Pharmaceutics Elsevier

Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers

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Publisher
Elsevier
Copyright
Copyright © 2012 Elsevier B.V.
ISSN
0378-5173
D.O.I.
10.1016/j.ijpharm.2011.12.059
Publisher site
See Article on Publisher Site

Abstract

Article history: Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to Received 11 November 2011 conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are Received in revised form released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present 14 December 2011 work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact Accepted 21 December 2011 of platelet lysate (PL) with cornea ulcers. Available online 10 January 2012 A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the Keywords: obtained formulation was able to quickly thermogelify at about 32 C and was characterized by good Growth factors mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the Platelet lysate vehicle and stable in the formulation up to 15 days of storage at 2–8 C. Cornea Wound healing In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) Thermogelling showed that the formulation enhanced cell growth and put in evidence a synergistic effect of

Journal

International Journal of PharmaceuticsElsevier

Published: Apr 15, 2012

References

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