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( hsc70 -12) originally designated as HSE is known by its partial cDNA (Ko et al., 1992), and it belongs to the hsc70 -1 and hsc70 -3 subgroup. Gene Structure and Polymerase Chain Reaction Genomic clones ...
, and flower but not detected in green or yellow siliques (Wu et al., 1994). Reverse transcriptase (RT)-PCR is a powerful method for expression analysis of gene families because amplification from mRNAs can ...
. Information on the expression profiles of plant Hsc70s /Hsp70s will help determine their roles in growth and development. A 73 kDa Hsc70 purified from the cytosolic fraction of mungbean seeds was found ...
) for fescue. Results from RT-PCR confirmed the differential expressions of these transcripts. The differential expressions of at least 11 of these genes were attributable to heat stress rather than ...
”). Expression of Genes by HL We applied a high degree of stringency to all aspects of the data collection and statistical analysis. First, RNA was extracted and pooled from over 50 plants for each ...
protoplasts cotransformed with the indicated HSF expression plasmids and the two reporters with promoter/leader fragments derived from the AtHsp70 and AtHsc70 encoding genes (see block diagrams at top). (D ...
hybridization with magnetic beads (SHMB) techniques to identify differentially expressed genes in N. attenuata by comparing mRNA from M. sexta-attacked plants with that from developmentally synchronized ...
translation under low temperature conditions. The HWB and conditioning treatments also increased the expression of a chaperonin gene ; these, like HSPs, are required to maintain protein structure and function ...
-induced gene expression leads to the rapid accumulation of heat stress proteins (Hsps). Many of them act as molecular chaperones with important functions not only for the protection of proteins against ...
in profiling gene expression in Arabidopsis (Zhu et al., 2001a). This genomic approach significantly extended our knowledge on wound-responsive genes ( from less than 100 reported using conventional methods ...
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