The role of afferents to the locus coeruleus in the handling stress-induced increase in the release of noradrenaline in the medial prefrontal cortex: a dual-probe microdialysis study in the rat brain

The role of afferents to the locus coeruleus in the handling stress-induced increase in the... This study was aimed to identify the neuronal pathways that mediate the handling stress-induced increase in the release of noradrenaline in the medial prefrontal cortex of the rat brain. For that purpose a microdialysis probe was implanted in the vicinity of the locus coeruleus and a second probe was placed in the ipsilateral medial prefrontal cortex. Receptor specific antagonists acting on the α 2 -adrenoceptor (50 μM idazoxan), GABA A (50 μM bicuculline), GABA B (100 μM (3,4-Dichlorophenyl)methyl)propyl)(diethoxymethyl) phosphonic acid; CGP 52432), acetylcholine (10 μM atropine), corticotropin releasing factor (CRF) (100 μM butyl-ethyl-(2,5-dimethyl-7-(2,4,6-trimethyl-phenyl)-7 H -pyrrolo(2,3- d )pyrimidin-4-yl)-amine; CP-154,526), NMDA glutamate (300 μM (±)-3(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid; CPP) and non-NMDA glutamate receptors (500 μM 6,7-dinitroquinoxaline-2,3-dione; DNQX) were infused into the locus coeruleus by retrograde dialysis, whereas extracellular noradrenaline was recorded in the ipsilateral medial prefrontal cortex. During infusion of the various compounds rats were gently handled for 10 min. Infusion of idazoxan potentiates the handling-induced increase in the release of noradrenaline in the medial prefrontal cortex. The infusions of, atropine, bicuculline, CGP 52432 and DNQX were without effect on the handling response. Infusion of the NMDA receptor antagonist CPP or the non-peptide CRF receptor antagonist CP-154,526 suppressed the stimulation of noradrenaline during stress. It is concluded that α 2 -adrenoceptors, NMDA glutamate receptors and CRF receptors modify the handling stress response of locus coeruleus neurones. The data suggest no major role for glutamatergic, GABAergic, or cholinergic afferents to the locus coeruleus in mediating the stress response. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Pharmacology Elsevier

The role of afferents to the locus coeruleus in the handling stress-induced increase in the release of noradrenaline in the medial prefrontal cortex: a dual-probe microdialysis study in the rat brain

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Publisher
Elsevier
Copyright
Copyright © 2000 Elsevier Science B.V.
ISSN
0014-2999
DOI
10.1016/S0014-2999(99)00793-1
Publisher site
See Article on Publisher Site

Abstract

This study was aimed to identify the neuronal pathways that mediate the handling stress-induced increase in the release of noradrenaline in the medial prefrontal cortex of the rat brain. For that purpose a microdialysis probe was implanted in the vicinity of the locus coeruleus and a second probe was placed in the ipsilateral medial prefrontal cortex. Receptor specific antagonists acting on the α 2 -adrenoceptor (50 μM idazoxan), GABA A (50 μM bicuculline), GABA B (100 μM (3,4-Dichlorophenyl)methyl)propyl)(diethoxymethyl) phosphonic acid; CGP 52432), acetylcholine (10 μM atropine), corticotropin releasing factor (CRF) (100 μM butyl-ethyl-(2,5-dimethyl-7-(2,4,6-trimethyl-phenyl)-7 H -pyrrolo(2,3- d )pyrimidin-4-yl)-amine; CP-154,526), NMDA glutamate (300 μM (±)-3(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid; CPP) and non-NMDA glutamate receptors (500 μM 6,7-dinitroquinoxaline-2,3-dione; DNQX) were infused into the locus coeruleus by retrograde dialysis, whereas extracellular noradrenaline was recorded in the ipsilateral medial prefrontal cortex. During infusion of the various compounds rats were gently handled for 10 min. Infusion of idazoxan potentiates the handling-induced increase in the release of noradrenaline in the medial prefrontal cortex. The infusions of, atropine, bicuculline, CGP 52432 and DNQX were without effect on the handling response. Infusion of the NMDA receptor antagonist CPP or the non-peptide CRF receptor antagonist CP-154,526 suppressed the stimulation of noradrenaline during stress. It is concluded that α 2 -adrenoceptors, NMDA glutamate receptors and CRF receptors modify the handling stress response of locus coeruleus neurones. The data suggest no major role for glutamatergic, GABAergic, or cholinergic afferents to the locus coeruleus in mediating the stress response.

Journal

European Journal of PharmacologyElsevier

Published: Jan 17, 2000

References

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